Next generation sequencer for the genetic analysis of pathogens affecting bee species

用于影响蜜蜂物种的病原体遗传分析的下一代测序仪

• 批准号: 474842-2014
• 负责人: Castillo, Carlos
• 金额: $ 10.93万
• 依托单位: Grande Prairie Regional College
• 依托单位国家: 加拿大
• 项目类别: Applied Research Tools and Instruments Grants
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=565108
• 关键词: Next; generation; sequencer; genetic; analysis

The National Bee Diagnostic Centre - TAC (Technology Access Centre) is the only state-of-the-art laboratory in Canada dedicated exclusively to diagnostics, training and applied research for bee species. We provide a comprehensive array of analyses for the beekeeping industry to determine the health of bee stock, whose results can be used for management purposes. In addition, the NBDC-TAC with the active participation of beekeepers performs applied research focused on bee pathologies. We also collaborate with Federal, Provincial, University and College researchers on studies of colony losses, disease dynamics and pathogens of wild bee species. Next Generation Sequencing (NGS) has changed, in recent years, the way laboratories perform genetic analyses and diagnostics. We are now able to identify several targets in a single sample within a single run. It is possible to perform a wide and deep screening of known diseases affecting an organism, and even identify new ones. The NBDC-TAC plans to employ NGS technology to track, maintain and increase our controls and constructs used for diagnostics; determine disease strains, identify new disease-producing organisms, and detect Single Nucleotide Polymorphism (SNPs) whose application to screen Africanised hybrids is extremely important for Canada international trade of honey bee stock. This genetic analysis in combination with pathogen quantification and localization on tissue sections will give us a more complete and clearer picture of diseases, symptoms and outbreaks. The project will improve in-house capability and performance transforming the scope for all our applied research by digging into genetic information not available before. The equipment perfectly complements NBDC-TAC services and research, being unique for a teaching institute in Northern Alberta. Students in the Peace Country and from other regions will be trained on specialized laboratory skills contributing to the formation of highly qualified personnel. In addition, we will be able to enter into new exciting applied research collaborations with AAFC, Universities and other national and international organizations.

国家蜜蜂诊断中心- TAC（技术访问中心）是加拿大唯一一个专门致力于蜜蜂物种诊断，培训和应用研究的最先进的实验室。我们为养蜂业提供了一系列全面的分析，以确定蜜蜂种群的健康状况，其结果可用于管理目的。此外，在养蜂人的积极参与下，国家生物多样性与发展中心-技术咨询中心开展以蜜蜂病理学为重点的应用研究。我们还与联邦、省、大学和学院的研究人员合作，研究野生蜜蜂物种的蜂群损失、疾病动态和病原体。近年来，下一代测序（NGS）改变了实验室进行遗传分析和诊断的方式。我们现在能够在一次运行中识别单个样品中的多个靶标。可以对影响生物体的已知疾病进行广泛而深入的筛查，甚至识别新的疾病。NBDC-TAC计划采用NGS技术来跟踪，维持和增加我们用于诊断的控制和构建;确定疾病菌株，识别新的致病生物，并检测单核苷酸多态性（SNP），其应用于筛选非洲化杂交种对加拿大蜜蜂的国际贸易非常重要。这种遗传分析与病原体定量和组织切片定位相结合，将使我们对疾病、症状和爆发有一个更完整和更清晰的了解。该项目将提高内部能力和性能，通过挖掘以前无法获得的遗传信息，改变我们所有应用研究的范围。该设备完美地补充了NBDC-TAC的服务和研究，是北方阿尔伯塔教学机构的独特之处。和平国家和其他区域的学生将接受专业实验室技能培训，以培养高素质的人才。此外，我们将能够与AAFC，大学和其他国家和国际组织进行新的令人兴奋的应用研究合作。