Elucidating roles for poplar SNF1-related protein kinases(SnRK1) in dormancy-associated processes

阐明杨 SNF1 相关蛋白激酶 (SnRK1) 在休眠相关过程中的作用

• 批准号: 311887-2011
• 负责人: Cooke, Janice
• 金额: $ 1.97万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=571642
• 关键词: Elucidating; roles; poplar; SNF1; related

Dormancy is an adaptation that temperate zone forest trees and other perennials have acquired to withstand the harsh conditions of winter. Dormancy is usually triggered by autumn-associated environmental cues such as shortened daylength and lower temperatures, and is accompanied by a suite of other developmental and physiological events that allow the plant to withstand inclement winter conditions, such as bud formation, cold, freezing and/or desiccation tolerance, and carbon and nitrogen resource reallocation and storage reserve synthesis. Late dormancy acquisition may lead to desiccation or cold injury, irrevocable loss of resources, or other negative consequences, while early dormancy acquisition abbreviates the growing season and reduces plant productivity. Molecular regulators of dormancy-associated processes are thus potential determinants of plant survival, growth, productivity, and fitness. It is surprising that only a few dormancy-associated molecular regulators have been identified to date in any plant species. The goal of my research program is to discover and characterize molecular regulators of processes implicated in dormancy acquisition and overwintering in forest trees. Since annuals cannot be used to study dormancy, poplars are ideal models for investigating dormancy-associated regulators. My laboratory has new evidence that a group of protein kinases called SnRK1 may be involved in regulating dormancy-associated processes in poplar. I propose to use a number of molecular biology and genomics techniques to discover SnRK1-regulated genes and proteins in poplar during dormancy acquisition, and use this information to identify processes that are regulated by SnRK1 during this transition from active growth to dormancy. I also aim to identify factors that regulate SnRK1. Together, this information will provide new insight into regulation of dormancy-associated processes in forest trees such as poplar.

休眠是温带森林树木和其他多年生植物为抵御冬季严酷条件而获得的一种适应。 休眠通常由与秋季相关的环境因素触发，例如日照缩短和温度降低，并伴随着一系列其他发育和生理事件，使植物能够承受严酷的冬季条件，例如芽形成、寒冷、冷冻和/或干燥耐受性，以及碳和氮资源重新分配和储存储备合成。较晚获得休眠可能会导致干燥或冷害、不可挽回的资源损失或其他负面后果，而过早获得休眠会缩短生长季节并降低植物生产力。因此，休眠相关过程的分子调节剂是植物生存、生长、生产力和适应性的潜在决定因素。令人惊讶的是，迄今为止在任何植物物种中只发现了一些与休眠相关的分子调节因子。我的研究计划的目标是发现和表征与森林树木休眠获取和越冬有关的过程的分子调节因子。由于一年生植物不能用于研究休眠，因此杨树是研究与休眠相关的调节因子的理想模型。 我的实验室有新证据表明，一组称为 SnRK1 的蛋白激酶可能参与调节杨树的休眠相关过程。 我建议使用多种分子生物学和基因组学技术来发现杨树在休眠获得过程中受 SnRK1 调控的基因和蛋白质，并利用这些信息来识别在从活跃生长到休眠的转变过程中受 SnRK1 调控的过程。 我还旨在确定调节 SnRK1 的因素。 总之，这些信息将为杨树等林木休眠相关过程的调控提供新的见解。