Regulating viral protein production and stability using small molecules

使用小分子调节病毒蛋白的产生和稳定性

• 批准号: 418719-2012
• 负责人: Banfield, Bruce
• 金额: $ 2.48万
• 依托单位: Queen's University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=573797
• 关键词: Regulating; viral; protein; production; stability

Viruses are remarkably selective and efficient nucleic acid delivery devices that serve as excellent model systems for studying complex protein assemblies as well as core cellular functions. Some of the most complicated and poorly understood viruses are the members of the Herpesviridae. In the case of the model herpesvirus studied in our laboratory, herpes simplex virus type 2, the viruses are built from about 100 different proteins. A focus of research in the Banfield laboratory is to understand the mechanisms by which these fascinating structures assemble. While much progress has been made in studying virus assembly, this progress has been hampered by the fact that many virus components are essential for the successful production of new virions. In this application we explore new approaches for regulating viral protein expression and stability that alleviates problems associated with contemporary procedures. To do this, we will combine the use of cutting edge genetic tools for the manipulation of herpesvirus genomes and two newly described methodologies for the pharmacological regulation of protein production. In sum, we propose to address a previously intractable problem in virus assembly by developing new technologies designed specifically to overcome inherent limitations of current state-of-the-art methodologies. If our approach is successful it is anticipated that our studies will have a profound impact on not only the ability of researchers to study the kinetics and regulation of virion assembly and protein function but will also provide additional tools for the analysis of other multiprotein complexes found in animal cells.

病毒是非常具有选择性和高效的核酸传递装置，是研究复杂蛋白质组装和核心细胞功能的优秀模型系统。一些最复杂和知之甚少的病毒是疱疹病毒科的成员。在我们实验室研究的疱疹病毒模型中，单纯疱疹病毒2型，病毒由大约100种不同的蛋白质构成。班菲尔德实验室的一个研究重点是了解这些迷人结构组合的机制。