UPLC-MSMS characterization and analysis of peptide fractionation from a biological lactose hydrolysis and marine by-product proteins

对生物乳糖水解和海洋副产物蛋白质的肽分级分离进行 UPLC-MSMS 表征和分析

• 批准号: 478693-2015
• 负责人: Bierenstiel, Matthias
• 金额: $ 1.82万
• 依托单位: Cape Breton University
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=579729
• 关键词: UPLC; MSMS; characterization; analysis; peptide

Saputo is a Canadian dairy food producer with a wide range of food products and has developed lactose-based fermentation and hydrolysis processes for proteins. The targeted peptides have applications in animal feed additives. The peptides have also health benefits for humans. In this confidential NSERC Engage project, Dr. Bierenstiel's research team will provide chemical expertise and state-of-the art equipment for rapid peptide analysis. The main equipment is an ultra performance liquid chromatograph with time-of-flight mass detector. UPLC is particularly suited for its enhanced separation, short separation times (< 5 min) and small sample quantities. Cape Breton University's Waters Xevo G2 UPLC-MSMS has a ToF detector for up to 2,000 m/z with elemental composition accuracy and quantitative capability. The quaternary solvent system with +/- modes for ESI and CI allows for excellent tailoring of analytical methods. The double focussed MSMS has a selective ion mode option (SIMS) that allows specific targeting of molecules/peptides in a complex matrix. In addition, the UPLC is connected to a photodiode array (PDA) detector allowing UV-vis profiling without mass restrictions. Results of rapid peptide analyses will be used for improvement and optimization of fermentation and protein hydrolysis process.

Saputo是一家加拿大乳制品食品生产商，拥有多种食品，并开发了以乳糖为基础的 蛋白质的发酵和水解过程。靶向肽在动物饲料中有应用 添加剂这些肽对人类也有健康益处。 在这个保密的NSERC Engage项目中，Bierenstiel博士的研究团队将提供化学专业知识， 用于快速肽分析的最先进设备。主要设备是超性能液体 具有飞行时间质量检测器的色谱仪。UPLC特别适用于其增强的分离，短 分离时间（< 5分钟）和少量样品。布雷东角大学的沃茨Xevo G2 UPLC-MSMS具有高达2，000 m/z的ToF检测器，具有元素组成准确度和定量分析能力。 能力。具有用于ESI和Cl的+/-模式的四元溶剂系统允许对ESI和Cl进行优异的定制。 分析方法双聚焦MSMS具有选择性离子模式选项（西姆斯）， 靶向复杂基质中的分子/肽。此外，UPLC连接到光电二极管阵列 (PDA)检测器允许紫外-可见光谱分析，没有质量限制。快速肽分析的结果将 用于发酵和蛋白质水解工艺的改进和优化。