UPLC-MSMS characterization and analysis of peptide fractionation from a biological lactose hydrolysis and marine by-product proteins

Saputo是一家加拿大乳制品生产商，拥有广泛的食品产品，并开发了以乳糖为基础的 蛋白质的发酵和水解过程。靶向多肽在动物饲料中有应用 添加剂。这些多肽对人类的健康也有好处。 在这个保密的NSERC Engage项目中，Bierenstiel博士的研究团队将提供化学专业知识和 最先进的多肽快速分析设备。主要设备是一种超性能液体 带有飞行时间质量检测器的色谱仪。UPLC特别适合于其增强的分离，短 分离时间(&lt；5分钟)和少量样品。布雷顿角大学的Waters Xevo G2 UPLC-MSMS具有高达2,000 m/z的TOF探测器，具有元素组成的准确性和定量 能力。具有用于ESI和CI的+/-模式的四元溶剂体系允许出色地剪裁 分析方法。双聚焦MSMS具有选择性离子模式选项(SIMS)，允许特定的 以复杂基质中的分子/多肽为靶标。此外，UPLC连接到光电二极管阵列 (PDA)探测器，允许无质量限制地进行UV-VIS分析。快速多肽分析的结果将是 用于发酵和蛋白质水解工艺的改进和优化。

Saputo is a Canadian dairy food producer with a wide range of food products and has developed lactose-based fermentation and hydrolysis processes for proteins. The targeted peptides have applications in animal feed additives. The peptides have also health benefits for humans. In this confidential NSERC Engage project, Dr. Bierenstiel's research team will provide chemical expertise and state-of-the art equipment for rapid peptide analysis. The main equipment is an ultra performance liquid chromatograph with time-of-flight mass detector. UPLC is particularly suited for its enhanced separation, short separation times (< 5 min) and small sample quantities. Cape Breton University's Waters Xevo G2 UPLC-MSMS has a ToF detector for up to 2,000 m/z with elemental composition accuracy and quantitative capability. The quaternary solvent system with +/- modes for ESI and CI allows for excellent tailoring of analytical methods. The double focussed MSMS has a selective ion mode option (SIMS) that allows specific targeting of molecules/peptides in a complex matrix. In addition, the UPLC is connected to a photodiode array (PDA) detector allowing UV-vis profiling without mass restrictions. Results of rapid peptide analyses will be used for improvement and optimization of fermentation and protein hydrolysis process.