UV-visible spectrophotometer for specific biochemical utility

用于特定生化用途的紫外-可见分光光度计

基本信息

批准号：
RTI-2016-00162
负责人：
Sen, Dipankar
金额：
$ 4.52万
依托单位：
Simon Fraser University
依托单位国家：
加拿大
项目类别：
Research Tools and Instruments
财政年份：
2015
资助国家：
加拿大
起止时间：
2015-01-01 至 2016-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=591364
关键词：
UV visible spectrophotometer specific biochemical

项目摘要

UV-Visible Absorption Spectroscopy is a powerful and versatile technique in biochemistry and biophysics, and a UV-Vis Spectrophotometer is an indispensable piece of equipment in any Molecular Biology / Biochemistry department, required not only for quantitation and characterization of nucleic acids and proteins, but also for the measurement of enzyme kinetics, structural transitions, and the interaction of ligands with bio-macromolecules. The students and faculty in the Simon Fraser University Department of Molecular Biology and Biochemistry have access to dedicated Fluorescence and Circular Dichroism (CD) spectrophotometers but not to a functional UV-Vis spectrophotometer. As a consequence, this strongly research-oriented department, comprising of ~30 faculty and > 100 graduate students, has no reliable access to such a fundamental piece of research equipment as a high quality UV-Vis spectrophotometer. Most critically, the research programs of the PI and co-applicants of this application are being hampered from lack access to a UV-Vis spectrophotometer that is able to meet the specifically tailored needs of these researchers in biochemistry and molecular biology, such as the use of radio-labeled samples, turbid samples, and samples containing live organisms, such as

紫外可见的吸收光谱是在生物化学和生物物理学方面是一种强大而多才多艺的技术，在任何分子生物学 /生物化学部门中，UV-VIS分光光度计是必不可少的设备，不仅需要用于定量和蛋白质的定量和特征，还需要用于核酸和蛋白质的启示，构建核酸和蛋白质的启动，均具有核酸剂，带有生物大分子的配体。西蒙·弗雷泽大学分子生物学和生物化学系的学生和教职员工可以使用专用的荧光和循环二色性分光光度计计，但不能获得功能性的UV-VIS分光光度计。结果，这个以研究为导向的强烈的部门，包括约30位教职员工和> 100名研究生，无法可靠地使用这样的基本研究设备，例如高质量的UV-VIS分光光度计。最重要的是，PI的研究计划和该应用程序的共同应用程序由于缺乏对紫外线分光光度计的访问而受到阻碍，该分光光度计能够满足这些研究人员在生物化学和分子生物学方面的特殊量身定制的需求，例如使用含有无线电标记的样品，浑浊的样品，浑浊的样品，浑浊的样品，浑浊的样品，浑浊的样品，以及