UV-visible spectrophotometer for specific biochemical utility

用于特定生化用途的紫外-可见分光光度计

• 批准号: RTI-2016-00162
• 负责人: Sen, Dipankar
• 金额: $ 4.52万
• 依托单位: Simon Fraser University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=591364
• 关键词: UV; visible; spectrophotometer; specific; biochemical

UV-Visible Absorption Spectroscopy is a powerful and versatile technique in biochemistry and biophysics, and a UV-Vis Spectrophotometer is an indispensable piece of equipment in any Molecular Biology / Biochemistry department, required not only for quantitation and characterization of nucleic acids and proteins, but also for the measurement of enzyme kinetics, structural transitions, and the interaction of ligands with bio-macromolecules. The students and faculty in the Simon Fraser University Department of Molecular Biology and Biochemistry have access to dedicated Fluorescence and Circular Dichroism (CD) spectrophotometers but not to a functional UV-Vis spectrophotometer. As a consequence, this strongly research-oriented department, comprising of ~30 faculty and > 100 graduate students, has no reliable access to such a fundamental piece of research equipment as a high quality UV-Vis spectrophotometer. Most critically, the research programs of the PI and co-applicants of this application are being hampered from lack access to a UV-Vis spectrophotometer that is able to meet the specifically tailored needs of these researchers in biochemistry and molecular biology, such as the use of radio-labeled samples, turbid samples, and samples containing live organisms, such as

紫外-可见吸收光谱是生物化学和生物物理学中功能强大且用途广泛的技术，紫外-可见分光光度计是任何分子生物学/生物化学部门不可或缺的设备，不仅用于核酸和蛋白质的定量和表征，还用于测量酶动力学，结构转变以及配体与生物大分子的相互作用。 西蒙弗雷泽大学分子生物学和生物化学系的学生和教师可以使用专用的荧光和圆二色性（CD）光谱仪，但不能使用功能性紫外-可见分光光度计。 因此，这个由约30名教师和100多名研究生组成的研究型部门无法可靠地获得高质量紫外可见分光光度计这样的基础研究设备。 最关键的是，本申请的主要研究者和共同申请人的研究计划受到阻碍，因为他们无法获得能够满足这些研究人员在生物化学和分子生物学方面的特定需求的紫外-可见分光光度计，例如使用放射性标记样品、浑浊样品和含有活生物体的样品，例如