Diagnostic aptamer selection for the beetle pheromone verbenone and its derivatives

甲虫信息素马鞭酮及其衍生物的诊断适体选择

• 批准号: 538629-2019
• 负责人: Sen, Dipankar
• 金额: $ 1.82万
• 依托单位: Simon Fraser University
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=680592
• 关键词: Diagnostic; aptamer; selection; beetle; pheromone

Infestation by a variety of beetle species has very negative economic and ecological impacts on the forestry industry of British Columbia as well as of Canada as a whole. A Burnaby, B.C., biotechnology company, eSenso Biotech, is interested in developing a variety of electronic sensing platforms for monitoring beetle pheromones, in this specific case the compound verbenone. Verbenone has been approved as a bark beetle anti-aggregation pheromone; this is important because bark beetles and mountain pine beetles are economically extremely damaging pests. Verbenone can be used with high efficacy in reducing attacks on pine forests by beetles, and this kind of treatment has been performed successfully on a variety of types of trees-- the results obtained indicating efficacies that are both tree-type- and verbenone dose-dependent. eSenso has under development a number of different detection platforms for verbenone and related compounds, but none so far that make use of the highly efficient, economic, and robust platforms that incorporate "DNA aptamers" (antibody-like, analyte-binding DNA sequences that are isolated from random-sequence DNA libraries using a powerful methodology known as "in vitro selection" or "SELEX"). My research program at Simon Fraser University has long specialized in SELEX methodologies, and we have over the years not only isolated and characterized a large number of highly diverse DNA and RNA aptamers and catalysts, but we have invented a unique approach for utilizing such aptamers in electrochemical sensing devices for the targeted antigens/analytes in question. This, our unique and SFU-patented methodology for designing highly sensitive, on-chip, electrochemical sensors incorporating DNA aptamers is known as "Deoxyribosensors" or "DORS". The eSenso company approached us to (1) obtain sensitive and high specificity DNA aptamer(s) against verbenone, and (2) design DORS devices incorporating such aptamers for sensing and quantitation of verbenone and related compounds. This initial research interaction is expected to lead to a fruitful long-term collaboration between eSenso Biotech and my laboratory.

各种甲虫物种的侵扰对不列颠哥伦比亚省以及整个加拿大的林业产业产生了非常负面的经济和生态影响。 Esenso Biotech的Burnaby，Bunaby，Bunaby，Bibotechnology Company，有兴趣开发各种电子传感平台，以监测甲虫的信息素，在这种特定情况下是化合物动词。动力酮已被批准为树皮甲虫抗聚集信息素。这很重要，因为树皮甲虫和山松甲虫在经济上是极其破坏性的害虫。动酮可用于减少甲虫对松树森林攻击的高疗效，并且这种治疗已成功地对各种树木进行了成功 - 获得的结果表明，既有效率既依赖于树型和杂种酮剂量。 Esenso正在开发许多用于动力酮和相关化合物的不同检测平台，但是到目前为止，都没有使用高效，经济和健壮的平台，这些平台结合了“ DNA Apatamers”（类似于抗体，分析物结合的DNA序列，这些序列与随机dna库相隔离，从随机dna库中隔离，并使用强有力的方法论使用了强大的方法论，即“ vestros”的实质性选择。 我在西蒙·弗雷泽大学（Simon Fraser University）的研究计划长期以来一直专门从事SELEX方法论，多年来，我们不仅隔离并表征了大量高度多样化的DNA和RNA适体和催化剂，而且我们发明了一种独特的方法，用于利用这种化学的抗原感应设备来用于目标抗原/分析中的电化学传感设备。这是我们独特且专注于SFU的方法，用于设计高度敏感的，芯片上的电化学传感器，这些传感器掺入DNA Aptamers被称为“ Deoxyribosensors”或“ Dors”。 Esenso公司向我们接触（1）对动词酮获得敏感且高的特异性DNA适体，以及（2）设计的DORS设备，这些设备融合了用于感应和定量的动词和相关化合物的适体。预计这种最初的研究互动将导致Esenso Biotech与我的实验室之间的富有成果的长期合作。