Diagnostic aptamer selection for the beetle pheromone verbenone and its derivatives

甲虫信息素马鞭酮及其衍生物的诊断适体选择

• 批准号: 538629-2019
• 负责人: Sen, Dipankar
• 金额: $ 1.82万
• 依托单位: Simon Fraser University
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=680592
• 关键词: Diagnostic; aptamer; selection; beetle; pheromone

Infestation by a variety of beetle species has very negative economic and ecological impacts on the forestry industry of British Columbia as well as of Canada as a whole. A Burnaby, B.C., biotechnology company, eSenso Biotech, is interested in developing a variety of electronic sensing platforms for monitoring beetle pheromones, in this specific case the compound verbenone. Verbenone has been approved as a bark beetle anti-aggregation pheromone; this is important because bark beetles and mountain pine beetles are economically extremely damaging pests. Verbenone can be used with high efficacy in reducing attacks on pine forests by beetles, and this kind of treatment has been performed successfully on a variety of types of trees-- the results obtained indicating efficacies that are both tree-type- and verbenone dose-dependent. eSenso has under development a number of different detection platforms for verbenone and related compounds, but none so far that make use of the highly efficient, economic, and robust platforms that incorporate "DNA aptamers" (antibody-like, analyte-binding DNA sequences that are isolated from random-sequence DNA libraries using a powerful methodology known as "in vitro selection" or "SELEX"). My research program at Simon Fraser University has long specialized in SELEX methodologies, and we have over the years not only isolated and characterized a large number of highly diverse DNA and RNA aptamers and catalysts, but we have invented a unique approach for utilizing such aptamers in electrochemical sensing devices for the targeted antigens/analytes in question. This, our unique and SFU-patented methodology for designing highly sensitive, on-chip, electrochemical sensors incorporating DNA aptamers is known as "Deoxyribosensors" or "DORS". The eSenso company approached us to (1) obtain sensitive and high specificity DNA aptamer(s) against verbenone, and (2) design DORS devices incorporating such aptamers for sensing and quantitation of verbenone and related compounds. This initial research interaction is expected to lead to a fruitful long-term collaboration between eSenso Biotech and my laboratory.

各种甲虫物种的侵扰对不列颠哥伦比亚省以及整个加拿大的林业产生了非常负面的经济和生态影响。A Burnaby，B.C.，生物技术公司eSenso Biotech有兴趣开发各种电子传感平台，用于监测甲虫信息素，在这种特定情况下，化合物马鞭草烯酮。马鞭草烯酮已被批准作为树皮甲虫抗聚集信息素;这是重要的，因为树皮甲虫和山松甲虫是经济上极具破坏性的害虫。马鞭草烯酮可以高效地用于减少甲虫对松林的攻击，并且这种处理已经成功地在各种类型的树木上进行-所获得的结果表明效力是树木类型和马鞭草烯酮剂量依赖性的。 eSenso已经开发了许多不同的马鞭草烯酮和相关化合物的检测平台，但迄今为止还没有一个使用高效、经济和稳健的平台，这些平台包含“DNA适体”（抗体样的、结合分析物的DNA序列，使用称为“体外选择”或“SELEX”的强大方法从随机序列DNA文库中分离出来）。 我在西蒙弗雷泽大学的研究项目长期专注于SELEX方法学，多年来，我们不仅分离和表征了大量高度多样化的DNA和RNA适体和催化剂，而且我们发明了一种独特的方法，用于在电化学传感设备中利用这种适体用于目标抗原/分析物。这种独特的SFU专利方法用于设计高灵敏度的片上电化学传感器，其中包含DNA适体，称为“脱氧核糖传感器”或“DORS”。eSenso公司与我们接洽，以（1）获得针对马鞭草烯酮的灵敏和高特异性DNA适体，以及（2）设计包含此类适体的DORS装置，用于感测和定量马鞭草烯酮和相关化合物。这一初步的研究互动预计将导致eSenso Biotech和我的实验室之间富有成效的长期合作。