Metabolic Profiling of Corn Silage

ENGAGE-LALLEMAND总结部分（21行） . 玉米青贮是基于植物水溶性碳水化合物在厌氧条件下由乳酸菌（LAB）自然发酵而成。影响青贮饲料保存效果的最重要的单一因素是青贮筒仓内的厌氧程度。青贮饲料的有氧变质是世界范围内农场盈利和饲料质量的一个重要问题。所有暴露在空气中的青贮饲料在出料过程中由于好氧微生物活动而变质。这些损失可能达到70%的存储干物质在周边地区的顶层和附近的侧壁的掩体和可消化的碳水化合物和有机酸部分的消耗。由于较低的营养价值和对动物性能的负面影响的风险，青贮饲料由于暴露于空气而腐败是不希望的，这也与潜在病原性或其它不希望的微生物的增殖和霉菌毒素污染有关 工业合作伙伴LALLEMAND希望了解生物化学因素，这些因素可以最大限度地减少由不需要的霉菌和细菌引起的玉米青贮饲料有氧变质的风险，以及玉米青贮饲料发酵对各种接种剂（如乳酸产生菌）的反应动力学。因此，LALLEMAND的主要重点是在玉米发酵过程的初始阶段的给定时间获得生物化学变化的快照（即，代谢谱），其在发酵过程的初始阶段响应于各种LAB接种物而发生。这种方法将提供LALLEMAND在给定时间给定样品的总生化组成或“青贮饲料代谢组”的信息。这种整体方法对公司很有吸引力，因为青贮饲料代谢物的高通量（HTP）检测和定量不仅具有成本和时间效益，而且具有高度的可重复性。

Summary section for ENGAGE-LALLEMAND (21 lines) . Corn ensiling is based on the natural fermentation of plant water-soluble carbohydrates by lactic acid bacteria (LAB) under anaerobic conditions. The most important single factor that can influence the preservation efficiency of forage ensiling is the degree of anaerobiosis reached in the completed silo. The aerobic deterioration of silages is a significant problem for farm profitability and feed quality world-wide. All silages exposed to air deteriorate as a result of aerobic microbial activity during feedout. These losses could reach 70% of the stored dry matter in the peripheral areas top layer and near the sidewalls of the bunkers and are related to the depletion of the digestible carbohydrate and organic acid fractions. Spoilage of silage due to exposure to air is undesirable, due to the lower nutritive value and to the risk of negative effects on animal performance, which are also connected to the proliferation of potentially pathogenic or otherwise undesirable microorganisms and mycotoxin contamination The industrial partner LALLEMAND wishes to understand the biochemical factors that would minimize the risk of aerobic deterioration of corn silage caused by unwanted molds and bacteria, and the dynamics of corn silage fermentation in response to various inoculants such as lactic acid producing bacteria. Thus, the main focus of LALLEMAND is to obtain, at a given time during the initial phase of corn fermentation process, a snapshot of the biochemical changes (i.e., metabolic profiles) that occur during the initial phase of the fermentation process in response to various LAB inoculants. This approach will provide LALLEMAND information on the total biochemical composition or "silage metabolome" of a given sample at a given time. This holistic approach is attractive to the company since the high-throughput (HTP) detection and quantification of silage metabolites is not only cost- and time-effective, but highly reproducible.