Biochemical profiling and activity of the botanical extract CELEXT07

Mondias Naturals Inc(MNI)的使命和愿景是将基于证据的植物抗菌产品商业化，用于住宅、有机和商业农产品市场。Mondias已经鉴定出包括CELEX07在内的几种草本植物提取物具有抗菌活性。以前的NSERC-ENGGE提案(申请号506182-16)涉及到工业伙伴MNI评估将植物提取物用作生物杀虫剂防治重要作物经济疾病的需要。MNI的主要目的是获得从白屈菜植物根部制备的植物水提取物(简称CELEX 07)对番茄、烟草和生菜幼苗的细菌和真菌的最低抑菌浓度(MIC)信息，并将CELEXT07与市场上现有的植物提取物产品(Yrow和百里香花提取物)进行比较。结果表明，1%浓度的CELEXT07土壤浸种对烟草和生菜幼苗的生长有促进作用(增加株高和重量)，比未处理植株或与CELEXT07联合使用的植株有促进生长的作用。有了这些知识，MNI热衷于通过NSERC Engage Plus解决以下问题和目标：(1)从根和叶器官中制备的CELEXT07的活性代谢产物是什么(2)确定与植物生长刺激(例如植物激素)和增加对选择性病原体的抗性(例如酚类)有关的签约代谢物。(3)开展大规模温室试验，评估CELEXT07根与叶提取物对生菜灰霉病和油菜黄单胞菌的抑制效果。上述目标的实施将为Mondias Naturals Inc.提供关于植物提取物CELEX07中活性代谢物的独立评估和科学证据，以及开发针对特定农产品市场的单一治疗型制剂的生物技术潜力。

The mission and vision of Mondias Naturals Inc (MNI) is to commercialize evidence-based botanicalantimicrobial products for the residential, organic and commercial agricultural markets. Mondias has identifiedseveral herb extracts including CELEX07 that have antimicrobial activity. The previous NSERC-ENGAGEproposal (Application number 506182-16) was related to the needs of the industrial partner MNI to evaluate theuse of plant extracts as biopesticides against economically important diseases of plant crops. The main focus ofMNI was to obtain information on the minimum inhibitory concentration (MIC) of their botanical aqueousextracts (BE) prepared from plant roots of Chelidonium majus, (referred to as CELEX 07) against bacterial andfungal pathogens of tomato, tobacco and lettuce young plants, and compare CELEXT07 efficacy to existingbotanical extracts products found on the market (Yarrow and Thyme flower extracts). The results confirmedthat CELEXT07 applied as soil drench at the MIC 1% concentration showed growth stimulatory effect(increased plant height and weight) of tobacco and lettuce young plants compared to non-treated plants orplants treated with a combined dose of yarrow and CELEXT07. Armed with this knowledge, MNI is keen toaddress the following questions and objectives through NSERC Engage Plus: (1) What are the activemetabolites of CELEXT07 prepared from root and leaf organs (2) Identify the signatory metabolite groupresponsible for growth stimulation of plants (e.g., phytohormones) and increased resistance (e.g., phenolics)against selective pathogens. (3) Carry out large-scale greenhouse experiments toevaluate the efficacy ofCELEXT07 extracts from root vs. leaf for disease suppression of Botrytis cinerea and Xanthomonascampestris, the causal agents of grey mould and bacterial leaf spots on lettuce, respectively.The execution of the above objectives will provide Mondias Naturals Inc. an independent assessment andscientific evidence on the active metabolite in the botanical extract CELEX07 as well as the biotechnologicalpotential to develop a monotherapy-type line of formulations targeted for specific agricultural markets.