Biochemical profiling and activity of the botanical extract CELEXT07

Mondias Naturals Inc （MNI）的使命和愿景是为住宅，有机和商业农业市场商业化基于证据的植物抗菌产品。Mondias已经确定了几种具有抗菌活性的草药提取物，包括CELEX07。先前的nserc - engage提案（申请号506182-16）与工业合作伙伴MNI的需求有关，以评估植物提取物作为生物农药的使用，以防止植物作物的重要经济疾病。mni的主要重点是获得从Chelidonium majus植物根中提取的植物水提取物（celex07）对番茄、烟草和生菜幼苗的细菌和真菌病原体的最低抑制浓度（MIC）信息，并将CELEXT07与市场上现有的植物提取物产品（蓍草和百里香花提取物）的功效进行比较。结果证实，与未处理或蓍草和CELEXT07联合剂量处理的幼苗相比，在最小浓度为1%时，CELEXT07作为土壤水剂对烟草和生菜幼苗的生长有促进作用（增加株高和株重）。有了这些知识，MNI热衷于通过NSERC Engage Plus解决以下问题和目标：(1)从根和叶器官制备的CELEXT07的活性代谢物是什么？(2)确定负责刺激植物生长的标志代谢物组（例如植物激素）和增加对选择性病原体的抗性（例如酚类物质）。(3)开展大规模温室试验，评价celext07根提取物与叶提取物对生菜灰霉病病原灰霉病菌灰霉病菌灰霉病菌灰霉病菌和细菌性叶斑病菌黄病菌（xanthomascampestris）的抑制效果。上述目标的实现将为Mondias Naturals Inc.提供关于植物提取物CELEX07中活性代谢物的独立评估和科学证据，以及开发针对特定农业市场的单一疗法型配方的生物技术潜力。

The mission and vision of Mondias Naturals Inc (MNI) is to commercialize evidence-based botanicalantimicrobial products for the residential, organic and commercial agricultural markets. Mondias has identifiedseveral herb extracts including CELEX07 that have antimicrobial activity. The previous NSERC-ENGAGEproposal (Application number 506182-16) was related to the needs of the industrial partner MNI to evaluate theuse of plant extracts as biopesticides against economically important diseases of plant crops. The main focus ofMNI was to obtain information on the minimum inhibitory concentration (MIC) of their botanical aqueousextracts (BE) prepared from plant roots of Chelidonium majus, (referred to as CELEX 07) against bacterial andfungal pathogens of tomato, tobacco and lettuce young plants, and compare CELEXT07 efficacy to existingbotanical extracts products found on the market (Yarrow and Thyme flower extracts). The results confirmedthat CELEXT07 applied as soil drench at the MIC 1% concentration showed growth stimulatory effect(increased plant height and weight) of tobacco and lettuce young plants compared to non-treated plants orplants treated with a combined dose of yarrow and CELEXT07. Armed with this knowledge, MNI is keen toaddress the following questions and objectives through NSERC Engage Plus: (1) What are the activemetabolites of CELEXT07 prepared from root and leaf organs (2) Identify the signatory metabolite groupresponsible for growth stimulation of plants (e.g., phytohormones) and increased resistance (e.g., phenolics)against selective pathogens. (3) Carry out large-scale greenhouse experiments toevaluate the efficacy ofCELEXT07 extracts from root vs. leaf for disease suppression of Botrytis cinerea and Xanthomonascampestris, the causal agents of grey mould and bacterial leaf spots on lettuce, respectively.The execution of the above objectives will provide Mondias Naturals Inc. an independent assessment andscientific evidence on the active metabolite in the botanical extract CELEX07 as well as the biotechnologicalpotential to develop a monotherapy-type line of formulations targeted for specific agricultural markets.