Biochemical profiling and activity of the botanical extract CELEXT07

Mondias Naturals Inc（MNI）的使命和愿景是将循证植物抗菌产品商业化，用于住宅，有机和商业农业市场。Mondias已经鉴定了几种草药提取物，包括具有抗菌活性的CELEX 07。先前的NSERC-ENGAGE提案（申请号506182-16）与工业合作伙伴MNI的需求有关，以评估植物提取物作为生物农药对植物作物经济上重要的疾病的使用。MNI的主要重点是获得从白屈菜植物根中制备的植物水提取物（BE）（称为CELEX 07）对番茄、烟草和生菜幼苗的细菌和真菌病原体的最小抑菌浓度（MIC）信息，并将CELEX 07的功效与市场上现有的植物提取物产品（西洋蓍和百里香花提取物）进行比较。结果证实，与未处理的植物或用组合剂量的西洋蓍和CELEXT 07处理的植物相比，以MIC 1%浓度作为土壤灌洗液施用的CELEXT 07显示出烟草和莴苣幼苗的生长刺激作用（增加的植物高度和重量）。有了这些知识，MNI热衷于通过NSERC Engage Plus解决以下问题和目标：（1）从根和叶器官制备的CELEXT 07的活性代谢物是什么？（2）确定对植物生长刺激负责的签名代谢物组（例如，植物激素）和增加的抗性（例如，酚类）对抗选择性病原体。(3)开展大规模温室试验，以评估CELEXT 07根和叶提取物对灰葡萄孢和野油菜黄单胞菌（分别是生菜灰霉病和细菌性叶斑病的病原体）的抑制效果。上述目标的实施将为Mondias Naturals Inc.关于植物提取物CELEX 07中活性代谢物的独立评估和科学证据，以及开发针对特定农业市场的单一疗法型制剂系列的生物技术潜力。

The mission and vision of Mondias Naturals Inc (MNI) is to commercialize evidence-based botanicalantimicrobial products for the residential, organic and commercial agricultural markets. Mondias has identifiedseveral herb extracts including CELEX07 that have antimicrobial activity. The previous NSERC-ENGAGEproposal (Application number 506182-16) was related to the needs of the industrial partner MNI to evaluate theuse of plant extracts as biopesticides against economically important diseases of plant crops. The main focus ofMNI was to obtain information on the minimum inhibitory concentration (MIC) of their botanical aqueousextracts (BE) prepared from plant roots of Chelidonium majus, (referred to as CELEX 07) against bacterial andfungal pathogens of tomato, tobacco and lettuce young plants, and compare CELEXT07 efficacy to existingbotanical extracts products found on the market (Yarrow and Thyme flower extracts). The results confirmedthat CELEXT07 applied as soil drench at the MIC 1% concentration showed growth stimulatory effect(increased plant height and weight) of tobacco and lettuce young plants compared to non-treated plants orplants treated with a combined dose of yarrow and CELEXT07. Armed with this knowledge, MNI is keen toaddress the following questions and objectives through NSERC Engage Plus: (1) What are the activemetabolites of CELEXT07 prepared from root and leaf organs (2) Identify the signatory metabolite groupresponsible for growth stimulation of plants (e.g., phytohormones) and increased resistance (e.g., phenolics)against selective pathogens. (3) Carry out large-scale greenhouse experiments toevaluate the efficacy ofCELEXT07 extracts from root vs. leaf for disease suppression of Botrytis cinerea and Xanthomonascampestris, the causal agents of grey mould and bacterial leaf spots on lettuce, respectively.The execution of the above objectives will provide Mondias Naturals Inc. an independent assessment andscientific evidence on the active metabolite in the botanical extract CELEX07 as well as the biotechnologicalpotential to develop a monotherapy-type line of formulations targeted for specific agricultural markets.