Exploring the chemistry of ferrocene bioconjugates.

探索二茂铁生物共轭物的化学性质。

• 批准号: RGPIN-2016-06122
• 负责人: Kraatz, HeinzBernhard
• 金额: $ 5.46万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=616110
• 关键词: Exploring; chemistry; ferrocene; bioconjugates.

This NSERC discovery grant proposal focuses on ferrocene-peptide conjugates, their properties in solution and on surfaces, including their electrochemical and materials properties. In particular, we are exploiting the ability of ferrocene peptides to associate to form gel materials based on a nanofibrillar architecture. Such gel materials incorporate large amounts of solvents in their matrix. In this research program, we are exploring factors leading to aggregation and gel formation, among them is the influence of peptide chirality and self-sorting behavior of ferrocene peptide conjugates. Our short-term objectives are to expand our discovery-based research of stimuli-responsive and self-sorting Fc-peptide gels to include both organo- and hydrogels and study the effects of chirality on gelation. This has potential implications with regards to our understanding of chemical evolution leading to early life, and may provide insight into why life (in most cases) prefers homochiral peptide systems, and how simple peptide systems organize into more complex structures. Furthermore, we will explore Fc-peptides for film formation on gold substrates and to study their electrochemical properties in an attempt to obtain quantitative information about electron transfer in peptides. The idea is to relate this information based on peptide models back to electron transfer in biological systems. And lastly, we will study the interaction of Fc-peptides with metal ions, specifically for the design of active sites of metalloproteins in solution and on surfaces and will attempt to create a working model for iron-sulfur proteins, including a peptide model for the Rieske protein. With a team of students and postdoctoral trainees, this research program will allow us to explore interactions and functions of biomolecules at the molecular level and thus aid in guiding the design of structurally well-defined molecules with known assembly and materials properties. As part of this research program, we will train graduate and undergraduate students in synthetic peptide chemistry, the characterization of materials and provide expert training in electrochemical methods, which will enable our team to contribute significantly to the scholarly activity of the University of Toronto.

这个NSERC的发现资助提案侧重于二茂铁-肽缀合物，它们在溶液和表面上的特性，包括它们的电化学和材料特性。特别是，我们正在利用二茂铁肽的能力，以形成基于纳米纤维结构的凝胶材料。这种凝胶材料在其基质中掺入大量溶剂。在本研究计划中，我们正在探索导致聚集和凝胶形成的因素，其中包括肽手性和二茂铁肽缀合物的自分选行为的影响。 我们的短期目标是扩大我们的发现为基础的研究刺激响应和自分选Fc-肽凝胶，包括有机和水凝胶和研究的影响，凝胶化的手性。这对我们理解导致早期生命的化学进化具有潜在的影响，并可能提供关于为什么生命（在大多数情况下）更喜欢同手性肽系统以及简单肽系统如何组织成更复杂结构的见解。此外，我们将探索Fc-肽的膜形成在金基板上，并研究其电化学性质，试图获得有关肽中的电子转移的定量信息。这个想法是将基于肽模型的信息与生物系统中的电子转移联系起来。最后，我们将研究Fc肽与金属离子的相互作用，特别是在溶液中和表面上金属蛋白活性位点的设计，并将尝试创建一个铁硫蛋白的工作模型，包括Rieske蛋白的肽模型。 通过一个由学生和博士后学员组成的团队，该研究计划将使我们能够在分子水平上探索生物分子的相互作用和功能，从而有助于指导具有已知组装和材料特性的结构明确的分子的设计。作为这项研究计划的一部分，我们将培养研究生和本科生在合成肽化学，材料的表征，并提供电化学方法的专家培训，这将使我们的团队显着贡献的学术活动的多伦多大学。