Electrochemical method for monitoring kinase activity

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Kinases are enzymes that are involved in regulating key cell functions. The reaction catalyzed by kinases is the transfer of a phosphate group from adenosine triphosphate to a hydroxy amino acid. Their activity is carefully controlled and uncontrolled activation of these enzymes has been linked to various forms of cancer. In our proposal, we make use of this fundamental reaction and propose a method for the rapid monitoring and profiling the kinase activity in cell and tissue extracts using. This screening method exploits our recent discovery of the protein kinase catalyzed transfer of a redox label from a novel redox-active analogue of adenosine triphosphate. This approach will enable the quantification of the kinase activity by electrochemical methods. In our approach, we make use of an electrochemical peptide array that has individually addressable gold pads, each having a peptide specific for a particular protein kinase, allowing us to monitor the activity of