Identifying structural features of viral RNA recognized by host helicases

鉴定宿主解旋酶识别的病毒 RNA 的结构特征

• 批准号: RGPIN-2017-04003
• 负责人: Patel, Trushar
• 金额: $ 2.26万
• 依托单位: University of Lethbridge
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=629987
• 关键词: Identifying; structural; features; viral; RNA

Viruses hijack the host cell machinery and recruit host proteins to aid viral replication. Considerable effort has been made to identify host proteins that interact with viral RNA/proteins. However, the biochemical and structural basis of viral RNA recognition by the members of the DEAD-box family, which support viral replication, is poorly understood. My long-term research goal is to study these key host helicases that are exploited by many viruses in order to gain detailed insights into the molecular mechanisms behind a broad spectrum of host helicases and viral RNA interactions. Despite the ATP-dependent unwinding of nucleic acids being the established role of DEAD-box helicases, recent studies demonstrate that helicases influence all major aspects of RNA metabolism and often play significant roles in viral replication. Particularly, the helicases DDX3X and DDX5 interact with the viral 3' untranslated region (3' UTR) of Japanese encephalitis virus, hepatitis C virus, and SARS coronavirus, and support their replication. My laboratory will investigate the structural basis of the interactions of DDX3X/DDX5 with 3' UTRs of members of the Flavivirus genus (

病毒劫持宿主细胞机器并招募宿主蛋白来帮助病毒复制。已经做出了相当大的努力来鉴定与病毒RNA/蛋白质相互作用的宿主蛋白。然而，支持病毒复制的DEAD-BOX家族成员识别病毒RNA的生化和结构基础尚不清楚。我的长期研究目标是研究这些被许多病毒利用的关键宿主解旋酶，以获得对广泛的宿主解旋酶和病毒RNA相互作用背后的分子机制的详细了解。尽管依赖于ATP的核酸解离是DEAD-box解旋酶的既定作用，但最近的研究表明，解旋酶影响RNA代谢的所有主要方面，并经常在病毒复制中发挥重要作用。特别是，解旋酶DDX3X和DDX5与乙脑病毒、丙型肝炎病毒和SARS冠状病毒的病毒3‘非翻译区(3’UTR)相互作用，并支持它们的复制。我的实验室将研究DDX3X/DDX5与黄病毒属成员3‘UTRs相互作用的结构基础(