Generational Effects of Chemical Stressors - Molecular Mechanisms of Altered Reproductive Performance

化学应激源的世代效应 - 改变生殖性能的分子机制

• 批准号: RGPIN-2017-04454
• 负责人: Wiseman, Steve
• 金额: $ 2.4万
• 依托单位: University of Lethbridge
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=630569
• 关键词: Generational; Effects; Chemical; Stressors; Molecular

Co-ordinated networks of gene expression are essential for regulation of sexual reproduction of teleost fishes. The epigenome, including the methylome, is a critical regulator of gene expression and is responsive to environmental stimuli. The methylome is reprogrammed during embryogenesis. Because it is mitotically stable, alterations to the methylome caused by exposure to environmental stimuli during embryogenesis can affect gene expression throughout development, and thus physiological processes such as reproduction. Because the methylome also is meiotically stable, reproductive by generations of fishes can be affected by ancestral alterations to the methylome of the male or female germ line. Thus, alterations of the methylome at very early developmental stages has the potential to cause long-term effects on reproduction. However, this is poorly understood in teleost fishes. The goal of the research described here is to characterize the methylome as a critical regulator of the reproductive performance of fishes exposed to endocrine disrupting chemicals. First (F1) generation fathead minnows (

基因表达的协调网络对于调节硬骨鱼类的有性生殖是必不可少的。表观基因组，包括甲基化组，是基因表达的关键调节因子，并对环境刺激作出反应。甲基化组在胚胎发生期间被重编程。因为它是有丝分裂稳定的，在胚胎发生过程中暴露于环境刺激引起的甲基化组的改变可以影响整个发育过程中的基因表达，从而影响生殖等生理过程。因为甲基化组也是减数分裂稳定的，所以鱼类的世代生殖可以受到雄性或雌性生殖系的甲基化组的祖先改变的影响。因此，在非常早期的发育阶段甲基化组的改变有可能对生殖造成长期影响。然而，这在硬骨鱼中知之甚少。这里描述的研究的目标是表征甲基化作为一个关键的调节器的生殖性能的鱼类暴露于内分泌干扰化学品。第一代（F1）黑头呆鱼（