Continued Evaluation of a Rapid Vertical Flow Assay Platform with Surface-Enhanced Raman Spectroscopy

使用表面增强拉曼光谱对快速垂直流分析平台进行持续评估

• 批准号: 508399-2017
• 负责人: Brosseau, Christa
• 金额: $ 0.91万
• 依托单位: Saint Mary's University
• 依托单位国家: 加拿大
• 项目类别: Engage Plus Grants Program
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=639312
• 关键词: Continued; Evaluation; Rapid; Vertical; Flow

Rapid diagnosis of human disease at the patient point-of-care is key to timely and effective treatment. Thisresearch project will explore the extent to which an advanced spectroscopic technique, known assurface-enhanced Raman spectroscopy (SERS) can be used to render a simple rapid vertical flow assay into amuch more powerful quantitative test. A quantitative rapid vertical flow assay, which this project seeks todemonstrate, would allow for earlier detection of human disease, and would allow for improved health caremonitoring (such as in determination of HIV viral load, etc). All of this would still be possible at the patientpoint-of-care, which would ensure rapid and effective disease treatment. Our recent Engage project is comingto an end, and we have made excellent progress on this front. Improved limit-of-detection and increasedselectivity have been illustrated, as well as guidelines for the production of stable and reproducible goldcolloids conjugated with both the capture agent and the Raman-active label. However, there is still much workto do, including pushing the limit of detection further through the exploration of other shapes / sizes ofparticles, as well as testing in biologically relevant matrices (i.e. serum). In addition, a robust data analyticsapproach is needed to advance the technology forward.

在患者的护理地点快速诊断人类疾病是及时有效治疗的关键。这项研究项目将探索一种先进的光谱技术，即众所周知的表面增强拉曼光谱(SERS)，可以在多大程度上将简单、快速的垂直流动分析转化为更强大的定量测试。本项目试图展示的定量快速垂直流动分析将有助于更早地发现人类疾病，并将有助于改进卫生保健监测(例如在确定艾滋病毒病毒载量等方面)。所有这一切在病人护理点仍然是可能的，这将确保快速和有效的疾病治疗。我们最近的Engage项目即将结束，我们在这方面取得了很好的进展。改进了检测限和提高了选择性，以及生产与捕捉剂和拉曼活性标记结合的稳定和可重现的金胶体的指导方针。然而，仍有许多工作要做，包括通过探索其他形状/大小的颗粒来进一步提高检测极限，以及在生物相关基质(即血清)中进行测试。此外，需要一种强大的数据分析方法来推动技术向前发展。