Novel method of in vivo gene delivery using a needle-free system

点击翻译按钮获取中文摘要

Recent advances in gene manipulation techniques have opened up new opportunities for the treatment ofhereditary diseases. Although remarkable progresses have been made in the development of the potential toolswhich can be used for gene editing in the living organisms, the methods to deliver these tools (e.g. foreignDNA molecules) inside the cells are still under-developed. In the current proposal we will use a needle-freedelivery system invented by a Canadian company that uses air pressure to deliver medicine deep inside thetissue. We will develop methodologies to deliver combination of DNA-chemical mixtures inside the cells. Thecombination of high air pressure together with DNA-chemical complexes has not been tried before. Ifsuccessful, this novel approach will expand the use of the existing system making it an essential tool in modernbiomedical research. This will not only benefit the research community, but also create scopes to expand thebusiness of the company and eventual contribution to Canadian Economy.