Novel method of in vivo gene delivery using a needle-free system

使用无针系统体内基因递送的新方法

• 批准号: 522020-2017
• 负责人: Murshed, Monzur
• 金额: $ 1.82万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=639464
• 关键词: Novel; method; vivo; gene; delivery

Recent advances in gene manipulation techniques have opened up new opportunities for the treatment ofhereditary diseases. Although remarkable progresses have been made in the development of the potential toolswhich can be used for gene editing in the living organisms, the methods to deliver these tools (e.g. foreignDNA molecules) inside the cells are still under-developed. In the current proposal we will use a needle-freedelivery system invented by a Canadian company that uses air pressure to deliver medicine deep inside thetissue. We will develop methodologies to deliver combination of DNA-chemical mixtures inside the cells. Thecombination of high air pressure together with DNA-chemical complexes has not been tried before. Ifsuccessful, this novel approach will expand the use of the existing system making it an essential tool in modernbiomedical research. This will not only benefit the research community, but also create scopes to expand thebusiness of the company and eventual contribution to Canadian Economy.

基因操作技术的最新进展为遗传性疾病的治疗开辟了新的机遇。尽管在开发可用于活体生物体基因编辑的潜在工具方面已经取得了显着进展，但在细胞内传递这些工具（例如外源 DNA 分子）的方法仍然不成熟。在当前的提案中，我们将使用一家加拿大公司发明的无针输送系统，该系统利用气压将药物输送到组织深处。我们将开发在细胞内传递 DNA 化学混合物组合的方法。高气压与 DNA 化学复合物的结合以前从未尝试过。如果成功，这种新颖的方法将扩大现有系统的使用范围，使其成为现代生物医学研究的重要工具。这不仅有利于研究界，而且还为扩大公司业务并最终为加拿大经济做出贡献创造了空间。