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Model directed phycocyanin over-production in blooming algal cell-factories

模型指导藻蓝蛋白在开花藻细胞工厂中过量生产

基本信息

批准号：
521681-2017
负责人：
Hallam, Steven
金额：
$ 1.82万
依托单位：
University of British Columbia
依托单位国家：
加拿大
项目类别：
Engage Grants Program
财政年份：
2017
资助国家：
加拿大
起止时间：
2017-01-01 至 2018-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=640491
关键词：
Model directed phycocyanin over production

项目摘要

Regulating the metabolic expression of photosynthetic microorganisms offers the possibility of producinghigh-value chemicals directly from sunlight, water and carbon dioxide. Despite a plethora of phenotypic andmolecular information, only a few cyanobacterial isolates have been developed into tractable genetic systemswith associated genome-scale metabolic models. This limits the number of strains that can be used as platformchemical producers. AlgaBloom International, a Vancouver-based biotech company specializing in bioreactordesign and cynaobacterial cell growth, is interested in developing their specific strain of Spirulina, optimizedfor high growth yields, as a platform chemical producer to extend their market range and profitability. Throughthe application of genome-scale metabolic modeling techniques and controlled laboratory growth experiments,this project aims to redirect metabolite flow through desired biosynthetic pathways in Spirulina. During thisphase of the project we will specifically focus on genome sequencing and model directed over-production ofphycocyanin, a valuable photosynthetic pigment with multiple applications in the food, medical andinstrumentation industries. Predicted overproduction phenotypes will be tested at both bench- and pilot-scalesand an optimized phycocyanin extraction and purification protocol will be developed for AlgaBloom.

调节光合微生物的代谢表达提供了直接从阳光、水和二氧化碳中生产高价值化学物质的可能性。尽管有大量的表型和分子信息，但只有少数蓝藻分离株已经发展成为具有相关基因组尺度代谢模型的可处理遗传系统。这限制了可以用作平台化学品生产者的菌株数量。AlgaBloom International是一家总部位于温哥华的生物技术公司，专门从事生物反应器设计和蓝藻细胞生长，该公司有兴趣开发其特定的螺旋藻菌株，优化其高生长产量，作为平台化学品生产商，以扩大其市场范围和盈利能力。通过基因组尺度代谢建模技术和受控实验室生长实验的应用，本项目旨在通过所需的生物合成途径重新定向螺旋藻的代谢物流动。在项目的这一阶段，我们将特别关注藻蓝蛋白的基因组测序和模型定向过度生产，藻蓝蛋白是一种有价值的光合色素，在食品、医疗和仪器仪表行业有多种应用。预测的过度生产表型将在实验和中试规模上进行测试，并将为AlgaBloom开发优化的藻蓝蛋白提取和纯化方案。