Development of a mass spectrometry-based foodomics approach to characterize the fate of selected antibiotic residues during food processing and storage.

开发基于质谱的食品组学方法来表征食品加工和储存过程中选定的抗生素残留物的命运。

• 批准号: RGPIN-2017-04800
• 负责人: Bayen, Stéphane
• 金额: $ 1.82万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=644834
• 关键词: Development; mass; spectrometry; based; foodomics

Despite the global efforts to manage antibiotics used as veterinary drugs, residues are frequently detected in food such as meat, seafood, milk, honey or eggs. Recently, we showed that one antibiotic, chloramphenicol, does not exist solely as the parent compound in food, but also occur as a multitude of (often unidentified) metabolites, and can transform during cooking into far more complex chemical mixtures with unknown impact for human health.***The long-term objective of this research program is to prove that many chemical contaminants are not solely present as the parent compound in food, but rather as a complex mixture of often unidentified structures as a result of thermal degradation/isomerization/interaction with food components. This program intends to develop a novel foodomics approach that improves the detection and identification of chemical contaminants in food from field to fork. This work will enrich the current database of known contaminants (e.g. veterinary drugs) with their metabolites and other structurally related chemicals (e.g. degradation products). While the present approach is applicable to any type of food, the initial efforts will focus on fish/seafood to demonstrate the conceptual approach.***The Short-term Objective of this program (5-years) is to characterize the fate of key veterinary drug residues in incurred seafood samples (tilapia/trout/lobster). The applicant intends to develop analytical methods for the non-targeted analysis of the metabolites of three veterinary drugs (nitrofurazone, oxytetracycline and sulfadimethoxine) in these fish/seafood (AIM 1); and investigate the changes induced by food storage and heating (cooking) on these residues (AIM 2). This research program will establish optimal foodomics protocols for antibiotics in seafood, thus paving the way for investigating other food matrices and contaminants (long-term objective).***This project will build innovation and deepen knowledge in the field of food chemistry, analytical chemistry and food safety. Notably, this study will allow developing analytical standards for seafood tissue metabolomics as standard protocols are urgently needed. The results of AIMs 1 and 2 will also highlight the diversity of metabolites and degradation by-products in food, which currently remains unidentified. Overall, this research will have an impact on the surveillance and the food safety risk assessment of veterinary drugs. It is anticipated to strengthen and complement the work of regulatory bodies in the identification and assessment of chemical risks.

尽管全球努力管理用作兽药的抗生素，但肉类、海鲜、牛奶、蜂蜜或鸡蛋等食品中仍经常发现残留物。最近，我们发现一种抗生素——氯霉素，不仅以母体化合物的形式存在于食品中，而且还以多种（通常未识别的）代谢物的形式存在，并且在烹饪过程中可以转化为更加复杂的化学混合物，对人类健康的影响未知。***该研究计划的长期目标是证明许多化学污染物不仅以母体化合物的形式存在于食品中，而且以通常未识别的复杂混合物的形式存在。 由于热降解/异构化/与食品成分相互作用而产生的结构。该计划旨在开发一种新颖的食品组学方法，改善从田间到餐桌的食品中化学污染物的检测和识别。这项工作将丰富当前已知污染物（例如兽药）及其代谢物和其他结构相关化学物质（例如降解产物）的数据库。虽然目前的方法适用于任何类型的食品，但最初的工作将集中在鱼类/海鲜上，以展示概念方法。***该计划的短期目标（5 年）是确定海鲜样品（罗非鱼/鳟鱼/龙虾）中关键兽药残留的去向。申请人打算开发对这些鱼类/海鲜中三种兽药（呋喃西林、土霉素和磺胺二甲氧嘧啶）代谢物进行非靶向分析的分析方法（AIM 1）；并研究食物储存和加热（烹饪）对这些残留物引起的变化（AIM 2）。该研究计划将为海鲜中的抗生素建立最佳的食品组学方案，从而为研究其他食品基质和污染物铺平道路（长期目标）。***该项目将在食品化学、分析化学和食品安全领域建立创新并加深知识。值得注意的是，由于迫切需要标准方案，这项研究将允许制定海鲜组织代谢组学的分析标准。 AIMs 1 和 2 的结果还将强调食品中代谢物和降解副产物的多样性，目前尚未确定。总体而言，本研究将对兽药监测和食品安全风险评估产生影响。预计将加强和补充监管机构在化学品风险识别和评估方面的工作。