Improving gene-editing protocols for application in swine production

改进基因编辑方案在养猪生产中的应用

• 批准号: 532394-2018
• 负责人: Bordignon, Vilceu
• 金额: $ 1.82万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=645828
• 关键词: Improving; gene; editing; protocols; application

Genome editing, the technology to turn on/off/correct genes at an embryonic stage of development, is viewed**by front-runners such as Alphagene-Olymel as "the key to the future", in terms of accelerated genetic**improvement and increased production efficiency. In fact, recent development of genome editing technologies**based on the CRISPR-Cas9 system has allowed the introduction of target changes in animal's genome in a**highly specific and efficient manner. For example, high value traits that are encoded by a single gene can now**be manipulated very efficiently and in a way that the result will be identical to mutations that occur randomly**in nature. Therefore, genome editing is expected to revolutionize livestock production, through the ability to**conduct highly specific genome modifications with impact on disease resistance, product quality, growth**efficiency, food conversion, and reproductive efficiency. However, there are many steps that need to be fully**characterized and optimized prior to achieving readiness for commercial applications. The proposed research**project will allow initiating the process of improving swine-specific gene editing protocols and will serve as a**first interaction between the company and the university, that is expected to evolve into a CRD grant**application for a more ambitious research collaboration. Canada is ranked 5th among world pork exporters,**totaling 3 billion dollars in 2011. Genome editing is anticipated to become the most efficient method to achieve**highly specific genome modifications with impact on livestock production efficiencies, exponentially faster**than traditional selective breeding. In that context, engineered endonucleases (e.g. CRISPR-Cas9) allow for**efficient, site-specific genome modifications that are regulatory and consumer-friendly, by simply turning on or**off specific endogenous genes, as opposed to introducing foreign DNA.

基因组编辑是一种在胚胎发育阶段打开/关闭/纠正基因的技术，被Alphagene-Olymel等领先者视为“未来的关键”，因为它可以加速遗传改良和提高生产效率。事实上，最近基于CRISPR-Cas9系统的基因组编辑技术 ** 的发展已经允许以 ** 高度特异性和有效的方式在动物基因组中引入靶向变化。例如，由单个基因编码的高价值性状现在可以被非常有效地操纵，并且结果与自然界中随机发生的突变相同。因此，基因组编辑有望通过进行高度特异性的基因组修饰来彻底改变畜牧生产，从而影响抗病性、产品质量、生长效率、食物转化和繁殖效率。然而，在实现商业应用之前，有许多步骤需要完全 ** 表征和优化。拟议的研究 ** 项目将允许启动改进猪特异性基因编辑协议的过程，并将作为公司和大学之间的第一次互动，预计将演变为CRD资助 ** 申请，以实现更雄心勃勃的研究合作。加拿大在世界猪肉出口国中排名第五，** 2011年出口总额为30亿美元。基因组编辑预计将成为实现高度特异性基因组修饰的最有效方法，对畜牧生产效率产生影响，比传统的选择性育种快得多。在这种情况下，工程化的核酸内切酶（例如CRISPR-Cas9）允许 ** 有效的、位点特异性的基因组修饰，这些基因组修饰是调节性的和消费者友好的，通过简单地打开或 ** 关闭特定的内源基因，而不是引入外源DNA。