Equipment to quantify levels of the pleiotropic, blood-based enzyme, thrombin

职务名称：用于定量多效性、基于血液的酶、凝血酶的水平的设备 ** 申请人的首要目标是催化和实现创新的研究方向和发现，以设计新的试剂来控制凝块形成，并改善用于例如血液制品储存和输血、用于血管内导管、移植物、心脏瓣膜、血液制品储存和输血的生物材料的血液生物相容性。生物合成和基于细胞的器官植入物、粘合剂和用于药物递送的纳米颗粒。实现这些目的的障碍是易逝的、有效的和多效性的酶凝血酶容易通过损伤或暴露于外来表面而产生，因此它诱导凝血和其他不期望的作用。为了设计抑制凝血酶生成或减轻其有害影响的方法，研究人员能够准确测量凝血酶生成是绝对必要的。本提案中要求的设备是校准的自动血栓图（CAT）分析仪，通常称为血栓镜，可准确定量血液、血浆或其他液体中产生的凝血酶水平。它已成为所有主要领先研究中心为此目的的标准工具。该设备利用了高灵敏度和特异性的荧光凝血酶底物。它对用户友好，适用于所有研究应用，并完全自动化用于高通量使用。市场上没有其他类似的仪器可以测量活性凝血酶。事实上，如果没有这一工具，申请人和其他用户就不得不使用费用更高、非标准化、容易出错和前后不一致的人工方法。这导致研究生产力的延迟，并赋予我们的研究和我们的学生竞争劣势。申请人是高素质，合作和国际上尊重他们的科学贡献。这些设备将位于UBC血液研究中心，位置方便，可供来自多个学院的许多研究小组使用，包括工程，科学，医学，制药科学和牙科。CAT分析仪将由一名高级全职硕士水平的研究助理管理，他还将提供HQP培训。我们预计，在该设备运行的前3年，将有150名以上的HQP接受培训。熟练使用该设备将为UBC学生的培训和就业前景提供显着优势。**************

Title: Equipment to quantify levels of the pleiotropic, blood-based enzyme, thrombin******The overarching goal of the applicants is to catalyse and enable innovative research directions and discoveries toward the design of new agents to control clot formation, and to improve blood biocompatibility of biomaterials used in, for example, blood product storage and transfusion, for intravascular catheters, grafts, heart valves, biosynthetic and cell-based organ implants, adhesives, and nanoparticles for drug delivery. An impediment to achieving these ends, is that the evanescent, potent and pleiotropic enzyme, thrombin, is readily generated by injury or exposure to foreign surfaces, whereupon it induces clotting and other undesirable effects. To design approaches that dampen the generation of thrombin or mitigate its deleterious effects, it is absolutely essential that researchers are able to accurately measure thrombin generation.******The equipment requested in this proposal, a Calibrated Automated Thrombogram (CAT) Analyzer, often referred to as a Thrombinoscope, accurately quantifies levels of thrombin generated in blood, plasma or other fluids. It has become the standard instrument for this purpose in all major leading research centres. The equipment takes advantage of a highly sensitive and specific fluorogenic thrombin substrate. It is user friendly, robust for all research applications and is fully automated for high-throughput use. There are no other similar instruments on the market that can measure active thrombin. Indeed, without this instrument, the applicants and other users are forced to use more costly, non-standardized, manual approaches that are prone to error and inconsistencies. This results in delays in research productivity and confers a competitive disadvantage to our research and to our students.******The applicants are highly qualified, collaborative and internationally respected for their scientific contributions. The equipment will be situated at the UBC Centre for Blood Research in an accessible location, available for use by many research groups from multiple faculties, including engineering, science, medicine, pharmaceutical sciences and dentistry. The CAT Analyzer will be managed by a senior full-time MSc level Research Assistant who will also provide training of HQP. We expect that >150 HQP will be trained on this equipment in the first 3 years of operation. Skilled use of this device will provide a significant advantage to UBC students in their training and employment prospects. **************