Equipment to quantify levels of the pleiotropic, blood-based enzyme, thrombin

标题:申请人的总体目标是催化和实现创新的研究方向和发现，以设计新的药物来控制凝块的形成，并改善用于血液制品储存和输血的生物材料的血液生物相容性，例如用于血管内导管，移植物，心脏瓣膜，生物合成和基于细胞的器官植入物，粘合剂，以及用于药物输送的纳米颗粒。实现这些目标的一个障碍是，易逝的、有效的和多效性的酶，凝血酶，很容易因损伤或暴露于异物表面而产生，从而引起凝血和其他不良影响。为了设计抑制凝血酶产生或减轻其有害影响的方法，研究人员能够准确测量凝血酶的产生是绝对必要的。******本提案中要求的设备是校准自动血栓图（CAT）分析仪，通常称为凝血镜，可准确量化血液、血浆或其他液体中产生的凝血酶水平。它已成为所有主要主要研究中心为此目的的标准仪器。该设备利用高灵敏度和特异性荧光凝血酶底物。它是用户友好的，强大的所有研究应用程序，是全自动的高通量使用。市场上没有其他类似的仪器可以测量活性凝血酶。事实上，如果没有这个工具，申请人和其他用户被迫使用更昂贵的、非标准化的、容易出错和不一致的手动方法。这导致了研究效率的延迟，并使我们的研究和学生处于竞争劣势。******申请人是高素质的，合作的，并因其科学贡献而受到国际尊重。该设备将位于UBC血液研究中心，可供来自多个院系的许多研究小组使用，包括工程，科学，医学，制药科学和牙科。CAT分析仪将由一名高级全职理学硕士水平的研究助理管理，他也将提供HQP培训。我们预计在前3年的操作中将有bbb150 HQP接受该设备的培训。熟练使用该设备将为UBC学生的培训和就业前景提供显着优势。**************

Title: Equipment to quantify levels of the pleiotropic, blood-based enzyme, thrombin******The overarching goal of the applicants is to catalyse and enable innovative research directions and discoveries toward the design of new agents to control clot formation, and to improve blood biocompatibility of biomaterials used in, for example, blood product storage and transfusion, for intravascular catheters, grafts, heart valves, biosynthetic and cell-based organ implants, adhesives, and nanoparticles for drug delivery. An impediment to achieving these ends, is that the evanescent, potent and pleiotropic enzyme, thrombin, is readily generated by injury or exposure to foreign surfaces, whereupon it induces clotting and other undesirable effects. To design approaches that dampen the generation of thrombin or mitigate its deleterious effects, it is absolutely essential that researchers are able to accurately measure thrombin generation.******The equipment requested in this proposal, a Calibrated Automated Thrombogram (CAT) Analyzer, often referred to as a Thrombinoscope, accurately quantifies levels of thrombin generated in blood, plasma or other fluids. It has become the standard instrument for this purpose in all major leading research centres. The equipment takes advantage of a highly sensitive and specific fluorogenic thrombin substrate. It is user friendly, robust for all research applications and is fully automated for high-throughput use. There are no other similar instruments on the market that can measure active thrombin. Indeed, without this instrument, the applicants and other users are forced to use more costly, non-standardized, manual approaches that are prone to error and inconsistencies. This results in delays in research productivity and confers a competitive disadvantage to our research and to our students.******The applicants are highly qualified, collaborative and internationally respected for their scientific contributions. The equipment will be situated at the UBC Centre for Blood Research in an accessible location, available for use by many research groups from multiple faculties, including engineering, science, medicine, pharmaceutical sciences and dentistry. The CAT Analyzer will be managed by a senior full-time MSc level Research Assistant who will also provide training of HQP. We expect that >150 HQP will be trained on this equipment in the first 3 years of operation. Skilled use of this device will provide a significant advantage to UBC students in their training and employment prospects. **************