Cell Imaging Multi-Mode Reader

细胞成像多模式读取器

• 批准号: RTI-2019-00420
• 负责人: DevriesAboud, Michaela
• 金额: $ 10.84万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=649172
• 关键词: Cell; Imaging; Multi; Mode; Reader

Accurately measuring the quantity and activities of metabolic and cell signalling processes is crucial in understanding physiological and pathological processes within the body. In order to conduct these types of analyses we require a multi-mode reader. Our current multi-mode reader is non-functioning and non-repairable since it is 20 years old, making acquisition of a new multi-mode reader an urgent matter. Acquisition of a multi-mode reader is vital to the success of our research programs and without this equipment our research programs would be dramatically limited. Acquisition of the Cytation 5 Cell Imaging and Multi-Mode Reader will not only allow us to conduct this vital research, but it will expand our current research capacity by allowing simultaneous quantification of metabolic and cell signalling pathways while also providing visualization of these cellular processes. This is important because subcellular location and the interaction between signaling components provide critical information on these complex and often highly integrated signaling pathways. Using the requested equipment we will be able to simultaneously quantify quantity, subcellular location and interaction of cell signaling components, dramatically increasing the richness of the data put out by our research programs. Importantly, the high quality components of the Cytation 5 yield highly accurate results, using simplified methods that are more cost effective than traditional analytical systems. Furthermore, the Gen5 software that accompanies the Cytation 5 system yields high quality images and precise data with an intuitive analysis platform. Using the Cytation 5, we will be able to expand on our understanding of the mechanisms that underpin fundamental physiological processes within tissues (muscle, liver and brain) as well as cells (muscle, liver, cancer), which will contribute substantially to our understanding of the regulation of muscle, liver, and brain metabolic function, and the regulation of cell death and differentiation. The Cytation 5 will support the research programs of 5 applicants within the Department of Kinesiology at the University of Waterloo. Furthermore, acquisition of this equipment will serve as a critical piece of infrastructure used to train the next generation of Canadian scientists. Direct access to the Cytation 5 will allow trainees to complete their analyses efficiently and give them the foundational and novel skills required to become leading researchers in both academic and industry careers. Overall, the acquisition of the Cytation 5 Cell Imaging and Multi-Mode Reader will greatly enhance the quantity and quality of the research we and our trainees are conducting and will increase our standing as research leaders in our fields in Canada.

准确测量代谢和细胞信号传导过程的数量和活动对于了解体内的生理和病理过程至关重要。为了进行这些类型的分析，我们需要一个多模式阅读器。我们目前的多模式阅读器是非功能性和不可修复的，因为它是20岁，使收购一个新的多模式阅读器是一个紧迫的问题。获得多模式阅读器对我们的研究计划的成功至关重要，如果没有这种设备，我们的研究计划将受到极大的限制。收购Cytation 5细胞成像和多模式阅读器不仅使我们能够进行这项重要的研究，而且还将通过同时量化代谢和细胞信号传导途径来扩展我们目前的研究能力，同时还提供这些细胞过程的可视化。这一点很重要，因为亚细胞定位和信号组分之间的相互作用为这些复杂且通常高度整合的信号通路提供了关键信息。使用所要求的设备，我们将能够同时量化数量，亚细胞位置和细胞信号成分的相互作用，大大增加了我们的研究计划提供的数据的丰富性。重要的是，Cytation 5的高质量组件使用比传统分析系统更经济高效的简化方法产生高度准确的结果。此外，Cytation 5系统附带的Gen5软件可通过直观的分析平台生成高质量图像和精确数据。使用Cytation 5，我们将能够扩展我们对组织（肌肉，肝脏和大脑）以及细胞（肌肉，肝脏，癌症）内基本生理过程的基础机制的理解，这将大大有助于我们对肌肉，肝脏和大脑代谢功能的调节以及细胞死亡和分化的调节的理解。Cytation 5将支持滑铁卢大学运动机能学系的5名申请人的研究项目。此外，购置这些设备将成为培训下一代加拿大科学家的关键基础设施。直接访问Cytation 5将使学员能够有效地完成分析，并为他们提供成为学术和行业职业生涯中领先研究人员所需的基础和新颖技能。总的来说，收购Cytation 5细胞成像和多模式阅读器将大大提高我们和我们的学员正在进行的研究的数量和质量，并将提高我们作为加拿大研究领域领导者的地位。