Analysis of cytokinin composition of raw materials and seaweed extract for the increased scope of product value

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Natural growth enhancers in crop production have gained a lot of interest in modern agriculture, especially in areas of organic plant production. Seaweeds and their extracts have been used for years in farming to improve plant growth and stress tolerance (Craigie, 2011). Although the unique, positive effects of seaweeds on plant performance have been widely reported, the exact mode of action of their active compounds is still poorly understood. Effective natural ingredients of bioinoculants include the phytohormones, and feature the Cytokinins (CKs). Cytokinins are critical factors responsible for promotion of plant growth and development as they up-regulate cell division in all parts of a plant (Sakakibara, 2006). Previous research revealed the presence of particular types of CKs in raw seaweed materials and in their derived products that are offered on the market (Stirk et al., 2003). However, early work looking at a narrow range of CK in Ascophyllum nodosum indicated that the detectable levels may not be sufficient for growth promotion of plants that receive the extract spray (Wally et al., 2013). The aim of this project is to employ new technology to expand the range of analyzed CKs including forms that have not been previously analysed in seaweed. This expanded analysis will be applied to a wider range of raw seaweed materials, commercial seaweed biofertilizers and extracts obtained through different production technologies at Acadian Seaplants Limited (ASL). In addition, we will analyse seaweed samples harvested during different seasons and from diverse geographical locations. Revealing the presence of the new types of CKs in seaweeds, and discovering the mechanisms of their biosynthesis and fate during processing and harvesting will enable ASL to better understand the further potential of their environmental-friendly products for growth promotion and crop quality improvement. It will also help to increase the scope of Company's value through the diversification of their product based on improved growth hormone effects. This ultimately, will facilitate ASL's response to specific consumer demands and increase possibilities for revenue in the Canadian horticulture and agriculture industry.****