Flow cytometer for analyses of host - pathogen interactions in aquatic vertebrates

流式细胞术是在单细胞水平上分析和定量细胞参数和细胞表面标志物的金标准。流式细胞仪可以定量测量各种细胞反应，如细胞活力、细胞周期阶段进展、多倍性分析、细胞活化状态、可溶性分子分泌，并且在通过免疫表型鉴定离散细胞群的能力方面是无与伦比的。BD Accuri C6流式细胞仪是台式流式细胞仪的最新进展，具有六个光学参数检测和增强的易用软件，可以以高通量格式同时捕获所有细胞数据。全面的数据采集允许即使是最新手的用户使用采集后的实验参数校准和调整来获取有意义的出版质量数据，从而使所有用户都可以使用该仪器。Katzenback研究小组将使用所要求的流式细胞仪仪器来研究加拿大重要水生脊椎动物中的宿主细胞-病原体相互作用，并发现抗菌肽的新的抗病毒方面。然而，这项研究受到滑铁卢大学或周边机构缺乏可用于申请人正在研究的生物安全二级病原体和病原体衍生产品的流式细胞仪的阻碍。购买流式细胞仪将使申请人能够进行必要的研究，同时为高素质的人员创造一个独特的培训场所，以发展流式细胞术方面令人垂涎的专业知识，实验设计荧光染料技术的基本知识，适当操纵病原体的生物安全培训以及新生物技术的发现和实施。流式细胞仪是高度通用的，被用于不同的学科，包括生物学，生物技术，制药和生物工程，并将有广泛的吸引力和研究人员，内部和外部的滑铁卢大学的用户，确保最大限度地利用该仪器。

Flow cytometry is the gold standard for analyses and quantification of cellular parameters and cell surface markers on a single-cell level. Flow cytometers can quantitatively measure various cellular responses such as cell viability, cell cycle stage progression, polyploidy analysis, cellular activation status, soluble molecule secretion and is unparalleled in the ability to identify discreet cell populations via immunophenotyping. The BD Accuri C6 flow cytometer is the latest advancement in bench top flow cytometry and has been developed with six optical parameter detection and enhanced, easy-to-use, software to capture all cellular data simultaneously in a high-throughput format. Wholistic data capture allows even the most novice user to acquire meaningful publication quality data using post-acquisition experimental parameter calibration and adjustment, making use of this instrument accessible to all users. The Katzenback research group will use the requested flow cytometer instrumentation to investigate host cell-pathogen interactions in important Canadian aquatic vertebrates and discover novel anti-viral facets of antimicrobial peptides. However, this research is hindered by the lack of flow cytometers at University of Waterloo or surrounding institutions available for use with the biosafety level II pathogens and pathogen-derived products being studied by the applicant. The acquisition of the flow cytometer will permit the applicant to conduct the necessary studies while creating a unique training ground for highly qualified personnel to develop coveted expertise in flow cytometry, fundamental knowledge of fluorescent dye technologies for experimental design, biosafety training for proper manipulation of pathogens and discovery to implementation of novel biotechnologies. Flow cytometers are highly versatile, being used across diverse disciplines including biology, biotechnology, pharmaceuticals and bioengineering and will have broad appeal and usership by researchers, both internal and external to the University of Waterloo, ensuring maximal utilization of the instrument.