Characterization of Airborne Cat Dander in a Cat Allergen (Fel d1) Exposure Chamber

红枫试验公司（渥太华，安大略省）创建了一个新的设施，有几个试验室，病人可以在那里接触过敏原（花粉和猫过敏原）。在该设施中，可以以可控的方式监测患者的症状（有或没有药物干预）。公司感兴趣的是获得更详细的过敏原（Fel d1附着在颗粒物上，通常是猫皮屑）的分散信息，以及及时监测颗粒物和浓度，以便在临床研究中更好地控制患者接触过敏原的一致性。空气中的猫皮屑浓度将使用TSI粒度分布分析仪进行现场测定，而与猫皮屑颗粒相关的空气中的fed1水平将通过安德森级联撞击器收集。同时，颗粒样品（使用Gillian 5000采样泵以稳定的流速提取**）将在玻璃纤维过滤器上收集。目前的**项目还将提供对Fel d1进行具有成本效益的ELISA分析的可行性研究。最后，对猫过敏原暴露室中的气溶胶扩散进行数值模拟，采用空气（初级流）的Reynolds平均Navier-Stokes （RANS）方程和颗粒相的Lagrangian随机游走模型。

Red Maple Trials (Ottawa, ON) created a new facility with several testing rooms, where patients can be**exposed to allergens (pollens and Fel d1 cat allergen). In this facility, the patient's symptoms (with or without**medication intervention) can be monitored in a controlled manner. The company is interested in obtaining more**detailed information regarding the dispersion of allergens (Fel d1 attached to particulate matter, normally cat**dander), and the monitoring of particulate matter and concentration in time, to better control the consistency of**exposure to allergens by the patients during clinical studies. Airborne cat dander concentration, will be**determined in-situ using a TSI Particle Size Distribution analyzer, while airborne Fel d1 levels associated to the**cat dander particles will be collected via an Anderson Cascade Impactor. In parallel, particle samples (extracted**using a Gillian 5000 sampling pump at a steady flow rate) will be collected on a glass fiber filters. The current**project will also provide the feasibility study of performing a cost-effective ELISA assaying of Fel d1. Finally,**numerical simulation of aerosol dispersion in the cat allergen exposure chamber will be performed using**Reynolds Averaged Navier-Stokes (RANS) equations for the air (primary flow) and Lagrangian random-walk**models for the particulate phase.