Bio-contained Bacillus subtilis for infection control

用于控制感染的生物枯草芽孢杆菌

• 批准号: 530175-2018
• 负责人: ODonoghue, Patrick
• 金额: $ 1.82万
• 依托单位: University of Western Ontario
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=661134
• 关键词: Bio; contained; Bacillus; subtilis; infection

The emerging and ongoing boom in the field of synthetic biology has led to a wealth of innovative solutions to meet diverse**industrial challenges. The biotechnology sector is heavily invested in engineering microorganisms, such as the bacterium Bacillus**subtilis, to create designer microbes that serve an industrially relevant purpose while remaining non-harmful to humans or the**equipment they are designed to act on.**Germiphene, a Brantford Ontario-based Biotech company has developed an evacuation system cleaner called Gobble. The**'active' ingredient in the product is a generally-regarded-as-safe (GRAS) B. subtilis strain that produces a biofilm that cleans and**deodorizes dental suction lines, preventing contamination with pathogenic bacteria. While effective, the product's designer**bacterial strain grows too quickly in the suction lines, causing clogging and filtration blockage, which requires expensive**remediation in addition to daily cleaning protocols in dental exam rooms.**Germiphene has requested that we develop a new B. subtilis strain in which the growth of the organism can be controlled by**addition or removal of an external and inert chemical supplement. Here we propose engineering a strain of B. subtilis that requires**an unnatural amino acid, which is not available in nature, as an essential nutrient for growth and biofilm production. By creating a**strain that depends on a nutrient that is not found in nature, we can prevent B. subtilis from overgrowing by limiting the supply of**the unnatural nutrient.**

合成生物学领域的新兴和持续繁荣带来了丰富的创新解决方案，以应对多样化的工业挑战。生物技术部门大量投资于工程微生物，如枯草芽孢杆菌 **，以创造设计微生物，用于工业相关目的，同时对人类或其设计作用的设备无害。位于安大略省的布兰特福德生物技术公司Germiphene开发了一种名为Gobble的疏散系统清洁剂。该产品中的"活性“成分通常被视为安全（GRAS）B。一种枯草杆菌菌株，产生生物膜，清洁和 ** 消毒牙科吸引线，防止病原菌污染。虽然有效，但该产品的设计者 ** 细菌菌株在抽吸管路中生长过快，导致堵塞和过滤堵塞，除了牙科检查室的日常清洁方案外，还需要昂贵的 ** 补救措施。杰米芬要求我们开发新的B。枯草杆菌菌株，其中生物体的生长可以通过添加或去除外部和惰性化学补充物来控制。在此，我们提出工程改造B的菌株。枯草芽孢杆菌需要一种非天然氨基酸，这是不存在于自然界中，作为生长和生物膜生产的必需营养素。通过创造一种依赖于自然界中没有的营养素的 ** 菌株，我们可以预防B。通过限制这种非天然营养素的供应来防止枯草杆菌过度生长。