Raman Spectroscopy Analysis to Improve the Validation of Cell Therapy Manufacturing Processes

拉曼光谱分析可改善细胞疗法制造工艺的验证

• 批准号: 508458-2017
• 负责人: Piret, James
• 金额: $ 11.48万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Collaborative Health Research Projects
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=662246
• 关键词: Raman; Spectroscopy; Analysis; Improve; Validation

Emerging cellular therapies have the potential to both greatly improve the health of individuals**and to reduce healthcare costs by curing diseases. For example, it has been established that**a cellular transplant therapy can reverse and potentially cure type 1 diabetes. However, there**are far too few cadaveric donors available relative to the many thousands of Canadians who**could benefit from this therapy. Generating pancreatic islets (insulin-generating cells) from**cultured stem cells is an exciting development that could provide an essentially unlimited**supply for transplantation into diabetic patients. It has already been demonstrated that these**cells can reverse diabetes in animal models, and a human clinical trial is currently underway.**However, several challenges must be overcome to make this cure for diabetes a reality.**Among them, improving the complex processes used to produce these cells is paramount.**For example, we need technologies capable of non-invasively (and without contact)**monitoring of the cultures so that the cells produced remain safe and effective. Our group has**shown that novel spectroscopic techniques can gather critical information using light scattered**by the cultured cells, tracking the critical developmental changes taking place as stem cells**become pancreatic cells. This project will advance this non-invasive technology so that it can**be used to assess and control the quality of stem cell-derived therapeutic cells destined for**transplantation. This will contribute to the development and approval of safe and efficacious**therapies with the potential of curing thousands of Canadians. While the first application will**target diabetes, our technology could also be applied to many other cell therapies that target**diseases such as cancer and heart disease cellular therapy.

新兴的细胞疗法有潜力极大地改善个人的健康**，并通过治愈疾病来降低医疗成本。例如，已经确定**细胞移植疗法可以逆转并有可能治愈 1 型糖尿病。然而，相对于成千上万可以从这种疗法中受益的加拿大人来说，可用的尸体捐赠者太少了。从培养的干细胞中产生胰岛（胰岛素生成细胞）是一项令人兴奋的发展，它可以为糖尿病患者的移植提供基本上无限的供应。已经证明，这些**细胞可以在动物模型中逆转糖尿病，目前正在进行人体临床试验。**然而，要使这种糖尿病疗法成为现实，必须克服几个挑战。**其中，改进用于生产这些细胞的复杂过程至关重要。**例如，我们需要能够非侵入性（且非接触式）**监测培养物的技术，以便产生的细胞保持安全和有效。我们的小组**表明，新颖的光谱技术可以利用培养细胞**散射的光来收集关键信息，跟踪干细胞**变成胰腺细胞时发生的关键发育变化。该项目将推进这种非侵入性技术，使其可用于评估和控制用于**移植的干细胞衍生治疗细胞的质量。这将有助于开发和批准安全有效的**疗法，有可能治愈数千名加拿大人。虽然第一个应用将**针对糖尿病，但我们的技术也可以应用于许多其他针对**疾病的细胞疗法，例如癌症和心脏病细胞疗法。