Raman Spectroscopy Analysis to Improve the Validation of Cell Therapy Manufacturing Processes

拉曼光谱分析可改善细胞疗法制造工艺的验证

• 批准号: 508458-2017
• 负责人: Piret, James
• 金额: $ 5.55万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Collaborative Health Research Projects
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=621912
• 关键词: Raman; Spectroscopy; Analysis; Improve; Validation

Emerging cellular therapies have the potential to both greatly improve the health of individualsand to reduce healthcare costs by curing diseases. For example, it has been established thata cellular transplant therapy can reverse and potentially cure type 1 diabetes. However, thereare far too few cadaveric donors available relative to the many thousands of Canadians whocould benefit from this therapy. Generating pancreatic islets (insulin-generating cells) fromcultured stem cells is an exciting development that could provide an essentially unlimitedsupply for transplantation into diabetic patients. It has already been demonstrated that thesecells can reverse diabetes in animal models, and a human clinical trial is currently underway.However, several challenges must be overcome to make this cure for diabetes a reality.Among them, improving the complex processes used to produce these cells is paramount.For example, we need technologies capable of non-invasively (and without contact)monitoring of the cultures so that the cells produced remain safe and effective. Our group hasshown that novel spectroscopic techniques can gather critical information using light scatteredby the cultured cells, tracking the critical developmental changes taking place as stem cellsbecome pancreatic cells. This project will advance this non-invasive technology so that it canbe used to assess and control the quality of stem cell-derived therapeutic cells destined fortransplantation. This will contribute to the development and approval of safe and efficacioustherapies with the potential of curing thousands of Canadians. While the first application willtarget diabetes, our technology could also be applied to many other cell therapies that targetdiseases such as cancer and heart disease cellular therapy.

新兴的细胞疗法有潜力极大地改善个人健康，并通过治愈疾病来降低医疗成本。例如，已经确定细胞移植疗法可以逆转并有可能治愈 1 型糖尿病。然而，相对于成千上万可以从这种疗法中受益的加拿大人来说，可用的尸体捐赠者太少了。从培养的干细胞中产生胰岛（胰岛素生成细胞）是一项令人兴奋的发展，它可以为糖尿病患者的移植提供基本上无限的供应。已经证明这些细胞可以在动物模型中逆转糖尿病，目前正在进行人体临床试验。然而，要使这种糖尿病疗法成为现实，必须克服一些挑战。其中，改进用于生产这些细胞的复杂过程至关重要。例如，我们需要能够非侵入性（且非接触式）监测培养物的技术，以便生产的细胞保持安全和有效。我们的小组已经证明，新型光谱技术可以利用培养细胞散射的光收集关键信息，跟踪干细胞变成胰腺细胞时发生的关键发育变化。该项目将推进这种非侵入性技术，使其可用于评估和控制用于移植的干细胞衍生治疗细胞的质量。这将有助于开发和批准安全有效的疗法，有可能治愈数千名加拿大人。虽然第一个应用将针对糖尿病，但我们的技术也可以应用于许多其他针对癌症和心脏病细胞治疗等疾病的细胞疗法。