Rapid nanopore-based single molecule counting for accurate concentration measurements

基于纳米孔的快速单分子计数可实现精确的浓度测量

• 批准号: 530554-2018
• 负责人: TabardCossa, Vincent
• 金额: $ 13.22万
• 依托单位: University of Ottawa
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=666358
• 关键词: Rapid; nanopore; based; single; molecule

Scientific instrumentation for Health and Life sciences, particularly diagnostics, is undergoing a revolution. New technologies have emerged that can interrogate individual molecules to enable important new opportunities in healthcare. Further progress toward the era of precision medicine requires the realization of two critical criteria. First, new tools will require ultrasensitivity, to enable detection of just a few copies of a target biomarker in a sample. Second, since the benefit of ultrasensitivity leading to early detection can be lost if the results are delayed and the opportunity to use those results for timely intervention is missed, these tools must also democratize detection, moving from a traditional centralized core lab to more rapid, mobile, low-cost testing on site. **Nanopore sensing is the most promising emerging ultrasensitive technology that is amenable to decentralization. Nanopores are inherently single molecule detectors and are entirely electrical in nature, with no need for complex, expensive, and bulky optical components and dyes. As such, they are uniquely suited to provide ultrasensitive detection in a miniaturized, portable and decentralized format.**In this proposal, we will address the main 'control points' that would enable the development of an ultrasensitive nanopore-based immunoassay technology amenable to the point of care, by increasing the counting speed of individual molecules to lower the limit of detection for accurate quantification of biomarkers. This project is highly interdisciplinary and uniquely positioned to advance the nanopore sensing field, and to strengthen the Canadian stake in this emerging area and in the larger nanobiotechnologies sector. Many students will be exposed to the challenges of collaboratively developing advanced technology for a rapidly-evolving market. The Canadian economy will also directly benefit as this and future innovations will be commercialized by our partner and other industry players. With this project, Canadians can look forward to better, faster, more precise, and more convenient healthcare, especially in remote settings.

健康和生命科学的科学仪器，特别是诊断仪器，正在经历一场革命。新技术已经出现，可以询问单个分子，从而为医疗保健提供重要的新机会。精准医疗时代的进一步发展需要实现两个关键标准。首先，新的工具将需要超灵敏度，以便能够检测样品中靶生物标志物的几个拷贝。其次，如果结果延迟，失去了利用这些结果进行及时干预的机会，那么超灵敏度导致早期检测的好处就会丧失，因此这些工具还必须使检测大众化，从传统的集中核心实验室转向更快速、移动的、低成本的现场检测。** 纳米孔传感是最有前途的新兴超灵敏技术，适合分散。纳米孔本质上是单分子检测器，本质上完全是电性的，不需要复杂、昂贵和庞大的光学组件和染料。因此，它们非常适合以小型化、便携式和分散式形式提供超灵敏检测。**在该提案中，我们将解决主要的“控制点”，这将使一个超灵敏的基于纳米孔的免疫测定技术的发展服从护理点，通过增加单个分子的计数速度，以降低检测的限制，准确定量的生物标志物。该项目是高度跨学科的，具有独特的地位，以推进纳米孔传感领域，并加强加拿大在这一新兴领域和更大的纳米生物技术领域的股份。许多学生将面临为快速发展的市场合作开发先进技术的挑战。加拿大经济也将直接受益，因为我们的合作伙伴和其他行业参与者将把这一创新和未来的创新商业化。通过这个项目，加拿大人可以期待更好、更快、更精确和更方便的医疗保健，特别是在偏远地区。