Development of a novel quantification method for microalgae in environmental water samples

环境水样中微藻定量新方法的开发

• 批准号: 533246-2018
• 负责人: Ward, Valerie
• 金额: $ 1.82万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=668203
• 关键词: Development; novel; quantification; method; microalgae

Current methods for quantify microalgae in water samples is based on chlorophyll content. However, different**species of algae and different water properties drastically affect the accuracy of these methods. Unfortunately,**the optical properties of chlorophyll make it very difficult to accurately measure the cell density. Other**established methods are not specific enough to distinguish microalgae from other types of aquatic organisms**like bacteria. Finally, highly accurate methods like cell counting is labour intensive and requires equipment that**cannot be carried in the field. While we often time of algae growing on beaches, microalgae can grow in many**different environments from industrial water cooling tanks, irrigation reservoirs, pool water, aquariums, or**aquaculture ponds where they can form blockages or create toxic environments for fish growth. Genemis**Laboratories Inc., the industrial partner in this work, specializes in rapid testing of water for microbial**contaminants with two major products, the AquaVial which is a visual limit test for a water sample's level of**microbial contamination, and AquaAssay, a quantitative test for microbial contamination testing for when a**time sensitive response is needed. Previous attempts to do so by Genemis were unsuccessful due to interference**with chlorophyll. This is an issue that has previously been resolved by Dr. Ward for other types of microalgae**quantification assays. This research will investigate a new rapid testing method for microalgae quantification**which will build on the existing products from Genemis. The Ward Lab's work on microalgae cultivation and**adapting analytical techniques to microalgae based applications makes them well suited to work with Genemis**in the development of this new product. Genemis is in an excellent position to quickly capitalize on the finding**of this work given their existing products for rapid bacterial testing.

目前对水样中微藻的定量方法是基于叶绿素含量。然而，不同种类的藻类和不同的水性质极大地影响了这些方法的准确性。不幸的是，**叶绿素的光学性质使得精确测量细胞密度变得非常困难。其他已建立的方法不够具体，无法区分微藻和细菌等其他类型的水生生物。最后，像细胞计数这样的高精度方法是劳动密集型的，需要**无法在现场携带的设备。虽然我们经常看到藻类在海滩上生长，但微藻可以在许多**不同的环境中生长，包括工业冷却池、灌溉水库、池水、水族馆或**水产养殖池塘，在这些环境中，微藻可能会形成堵塞或造成鱼类生长的有毒环境。Genemis**实验室公司是这项工作的工业合作伙伴，专门从事水中微生物**污染物的快速测试，有两种主要产品，AquaVial是对水样**微生物污染水平的视觉极限测试，AquaAssay是一种微生物污染定量测试，用于在需要**时间敏感响应时进行微生物污染测试。Genemis之前的尝试由于对叶绿素的干扰**而没有成功。这是沃德博士之前在其他类型的微藻定量分析中解决的问题。这项研究将在Genemis现有产品的基础上，研究一种新的微藻定量快速检测方法。沃德实验室在微藻培养和**将分析技术应用于微藻应用方面的工作使他们非常适合与Genemis**合作开发这一新产品。Genemis处于一个非常有利的地位，可以迅速利用这项工作的发现**，因为他们现有的产品可以进行快速细菌测试。