Application of targeted transcriptomics for determining early nutrient sediment retention capacity (NSRC)****

应用靶向转录组学测定早期养分沉积物保留能力 (NSRC)****

• 批准号: 521430-2018
• 负责人: Weisener, Christopher
• 金额: $ 16.15万
• 依托单位: University of Windsor
• 依托单位国家: 加拿大
• 项目类别: Strategic Projects - Group
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=668364
• 关键词: Application; targeted; transcriptomics; determining; early

Nutrient mitigation and their interception is a primary goal of source water protection and a main objective for effective sewage treatment, engineered water retention basins and agricultural best practice. Emphasis is often placed on monitoring nutrient sources and concentrations in water. However, the risks associated with nutrients mobilization from long-term sequestration in sediment reservoirs are not well understood in lakes, wetlands, and engineered systems (e.g. retention ponds, constructed wetlands, drainage ditches). These sediment-nutrient dynamics can significantly impact internal nutrient loading in shallow eutrophic systems and can cause delayed recovery after external loads have been abated. Nutrient cycling in sediment is driven largely by microbial processes, either directly by conversion of bound-nutrients to bioavailable forms or indirectly by regulating the physical/chemical conditions that control nutrient speciation and solubility. Classic approaches to measuring nutrient flux from sediments often overlook the dynamic and complex set of microbial pathways involved in regulating sediment nutrient retention capacity. With our Collaborators and Industrial Partners, this project will develop a rapid and cost effective technology to diagnose surficial sediment nutrient retention capacity as it relates to microbial gene expression (function) and microbial community composition. Specifically, this project will develop a targeted transcriptomic assay system capable of detecting diagnostic microbial gene expression profiles indicative of active nutrient flux and can serve as a proxy for more expensive and laborious classic chemical approaches. The assay will be system designed by generating novel gene expression libraries calibrated to in-situ microbial communities derived from several sediment environments including agricultural tributaries/drainage ditches, engineered retention systems and tributaries receiving wastewater treatment effluents. Such information will provide new insights for evaluating the health/potential risk of sediment-nutrient release leading to internal load, a current major concern for Lake Erie and a anticipated major stress vector in other Canadian and global watersheds related to climate change.

养分缓解及其拦截是源防水的主要目的，也是有效污水处理，工程水分保留盆地和农业最佳实践的主要目标。通常将重点放在监测水中的养分和浓度上。但是，在湖泊，湿地和工程系统中，人们对与沉积物储层长期隔离相关的营养动员的风险尚不清楚（例如，保留池塘，建造的湿地，排水沟渠）。这些沉积物 - 营养素动力学可以显着影响浅氟化系统中的内部营养负荷，并减轻外部负载后会导致延迟恢复。沉积物中的养分循环主要由微生物过程驱动，直接通过结合成分转化为生物利用形式，或通过调节控制养分的物质形成和溶解度的物理/化学条件而间接驱动。测量沉积物的营养通量的经典方法通常会忽略调节沉积物养分保留能力的动态和复杂的微生物途径。与我们的合作者和工业合作伙伴一起，该项目将开发一种快速且具有成本效益的技术，以诊断与微生物基因表达（功能）和微生物社区组成有关的表面沉积物养分保留能力。具体而言，该项目将开发一个有针对性的转录组测定系统，能够检测指示有效营养通量的诊断微生物基因表达曲线，并可以作为更昂贵且费力的经典化学方法的代理。该测定法将是通过生成新的基因表达文库设计的系统，这些基因表达文库校准了从几种沉积物环境中得出的原位微生物群落，包括农业支流/排水沟，工程性保留系统和收到废水处理废水的支流。这些信息将提供新的见解，以评估导致内部负载的沉积物 - 释放的健康/潜在风险，目前对伊利湖的主要关注点以及与气候变化有关的加拿大和全球其他分水岭预期的主要压力向量。