Genome sequencing and comparative analysis of brewing yeast cultures

酿酒酵母培养物的基因组测序和比较分析

• 批准号: 543995-2019
• 负责人: Mcconkey, Brendan
• 金额: $ 1.61万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=678933
• 关键词: Genome; sequencing; comparative; analysis; brewing

Homebrewing and craft brewing represent an increasingly large portion of the beer industry in Canada and internationally. As a result of the increase in small scale, custom, and craft brewing there is an increased demand for both mixed yeast cultures and characterization of strains within mixed cultures associated with particular styles and flavours. This project will profile different mixed cultures of yeast through genomic DNA sequencing to identify genes and genotypes associated with particular flavours and properties within mixed cultures, along with properties that allow for stable growth and predictable composition. Additionally, the project will develop a sequencing-based methodology to track the long-term stability of mixed yeast cultures as a quality control step to ensure that mixed culture yeast products are consistent over time. Sequencing will be done using Illumina and Oxford Nanopore Technology (ONT) sequencing. This project will assess ONT sequencing as a possible in-house resource for microorganism sequencing, potentially providing an affordable long-read sequencing platform with a rapid turnaround time and a much lower capital cost than other sequencing technologies.

家庭酿造和手工酿造在加拿大和国际啤酒行业中所占的比例越来越大。由于小规模、定制和手工酿造的增加，对混合酵母培养物和与特定风格和风味相关的混合培养物中的菌株的表征的需求增加。该项目将通过基因组DNA测序分析不同的酵母混合培养物，以确定与混合培养物中特定风味和特性相关的基因和基因型，沿着允许稳定生长和可预测成分的特性。此外，该项目将开发一种基于测序的方法，以跟踪混合酵母培养物的长期稳定性，作为质量控制步骤，以确保混合培养酵母产品随时间的推移保持一致。将使用Illumina和Oxford Nanopore Technology（ONT）测序进行测序。该项目将评估ONT测序作为微生物测序的可能内部资源，可能提供一个负担得起的长读段测序平台，具有快速的周转时间和比其他测序技术低得多的资本成本。