Identification of key regulatory elements of fruit ripening in grapevine (Vitis vinifera L.)

葡萄果实成熟关键调控元件的鉴定（Vitis vinifera L.）

• 批准号: RGPIN-2015-04760
• 负责人: Castellarin, Simone
• 金额: $ 1.75万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=679772
• 关键词: Identification; key; regulatory; elements; fruit

In Canada, the grape and wine industry has grown significantly in the past few decades, gaining both domestic and international recognition. The economic value of grapes is strongly related to fruit quality, which is determined by a plethora of compounds that accumulate during fruit ripening. Ripening represents a summation of processes that include molecular, chemical and physical changes. These processes are finely regulated by climatic conditions.***The goal of my research program is to identify the underlying biological mechanisms (key genes and metabolites) that trigger and regulate ripening in the fruit tissues, as well as the molecular pathways that transduce environmental signals within the plant and modulate cell metabolism and fruit quality.***My team of students and I will evaluate field and culture treatments to understand which solutes (i.e. sugars, abscisic acid) originating from the plant or synthesized in the berry itself are necessary to induce the major ripening processes (fruit softening, accumulation of pigments and volatile organic compounds) that determine fruit quality. The molecular pathways affected by these treatments will be identified and isolated in order to identify key genes involved in the signaling pathway. The effect of environmental factors such as temperature and light in regulating the level of sugars, abscisic acid and the key signaling genes will be investigated through open field and greenhouse experiments where these factors will be manipulated, and the fruit response will be evaluated at the gene expression and metabolite level. ***This program will combine robust field and laboratory experiments with a systems-level characterization of developing fruit, also utilizing advanced platforms for transcriptome (RNA-seq) and metabolite (GC-MS, LC-MS, HPLC) analyses. The knowledge generated, integrated with subsequent applied experiments, will benefit grape growers in BC, Ontario and other parts of Canada by developing new practices for improving fruit ripening and optimizing fruit quality in vineyards. ***Finally, the program will provide an excellent training platform in viticulture for MSc, PhD and undergraduate students. These HQP will learn and profit from the multidisciplinary nature of this program, which will provide training opportunities to develop research skills in plant biology and genomics, while also advancing knowledge in the field of applied physiology.**

在加拿大，葡萄和葡萄酒行业在过去几十年中发展迅速，获得了国内和国际的认可。葡萄的经济价值与果实质量密切相关，而果实质量是由果实成熟过程中积累的大量化合物决定的。成熟是一系列过程的总和，包括分子、化学和物理变化。这些过程受到气候条件的精细调节。*我的研究计划的目标是确定触发和调节果实组织成熟的潜在生物学机制（关键基因和代谢物），以及抑制植物内环境信号并调节细胞代谢和果实质量的分子途径。我和我的学生团队将评估田间和培养处理，以了解哪些溶质（即糖，脱落酸）来自植物或在浆果本身合成是必要的，以诱导主要成熟过程（水果软化，色素和挥发性有机化合物的积累），决定水果质量。受这些治疗影响的分子通路将被鉴定和分离，以鉴定参与信号通路的关键基因。环境因素如温度和光照在调节糖、脱落酸和关键信号基因水平方面的影响将通过开放田间和温室实验进行研究，在这些实验中将操纵这些因素，并在基因表达和代谢水平上评估果实的反应。* 该计划将联合收割机强大的现场和实验室实验与发育水果的系统级表征相结合，还利用先进的转录组（RNA-seq）和代谢物（GC-MS，LC-MS，HPLC）分析平台。所产生的知识，与随后的应用实验相结合，将有利于不列颠哥伦比亚省，安大略和加拿大其他地区的葡萄种植者，通过开发新的实践来改善葡萄园的果实成熟和优化果实质量。* 最后，该计划将为硕士，博士和本科生提供葡萄栽培的优秀培训平台。这些HQP将学习并从该计划的多学科性质中获益，该计划将提供培训机会，以发展植物生物学和基因组学的研究技能，同时还将推进应用生理学领域的知识。