Characterization of bioactive natural products from beneficial agricultural bacteria.

来自有益农业细菌的生物活性天然产物的表征。

• 批准号: 539275-2019
• 负责人: Sorensen, John
• 金额: $ 1.82万
• 依托单位: University of Manitoba
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=680348
• 关键词: Characterization; bioactive; natural; products; beneficial

Wheat is Canada's largest crop and the single biggest export earner of all our agricultural products. Soil and stubble born fungal diseases of wheat and canola are recognized as one of the main obstacles for the increase in production in Canada and around the world. In 2014, KGS Group isolated ten (10) unique bacteria strains from Manitoba soils. During 2016-2018, laboratory, greenhouse and field testing showed a remarkable ability for the strains to enhance growth in wheat, and then canola. In 2018, University of Manitoba and KGS Group personnel carried out several laboratory tests on the strains, discovering that three (3) KGS strains have antifungal properties.This project will develop novel chemical technology to optimize the extraction of the novel bioactive molecules from the KGS Group strains of bacteria. The KGS strains will be prepared and grown in the Sorensen lab using well established protocols. In addition, The complete characterization of the chemical structure of the isolated molecules will be carried out using a combination of spectroscopic techniques (NMR, and MS). Subsequent to this the antifungal activity assays will also be carried out in the Sorensen lab using existing techniques. Dr. John Sorensen is an expert in natural products chemistry with a particular focus on bioactive molecules produced by microorganisms. Dr. Stan Lozecznik from KGS group will be involved in all aspects of this project in an ongoing basis through a number of regularly scheduled meetings.Understanding the molecular basis for the plant growth promoting effects of the proprietary KGS strains in wheat and canola is critical towards commercialization and market development for these beneficial antifungal strains.This project will help KGS Group develop novel crop protection products for the agricultural sector. This will be essential for the diversification of the services offered by KGS Group.

小麦是加拿大最大的作物，也是我们所有农产品中最大的出口创汇产品。小麦和油菜的土壤和根茬真菌病害被认为是加拿大和世界各地增产的主要障碍之一。2014年，KGS集团从马尼托巴土壤中分离出十（10）种独特的细菌菌株。2016-2018年期间，实验室、温室和田间测试显示，这些菌株具有显着的促进小麦生长的能力，然后是油菜。2018年，马尼托巴大学和KGS集团人员对菌株进行了多次实验室测试，发现三（3）种KGS菌株具有抗真菌特性。该项目将开发新的化学技术，以优化从KGS集团菌株中提取新型生物活性分子。KGS菌株将使用完善的方案在Sorensen实验室中制备和生长。此外，将使用光谱技术（NMR和MS）的组合对分离分子的化学结构进行完整表征。此后，还将在Sorensen实验室使用现有技术进行抗真菌活性试验。John Sorensen博士是天然产物化学方面的专家，特别关注微生物产生的生物活性分子。KGS集团的Stan Lozecznik博士将通过定期会议参与该项目的各个方面。了解KGS专利菌株在小麦和油菜中促进植物生长的分子基础对于这些有益的抗真菌菌株的商业化和市场开发至关重要。该项目将帮助KGS集团开发新型作物保护产品，农业部门。 这对KGS集团提供的服务多样化至关重要。