Supercritical CO2 extraction of terpenoids from Cascade hops

啤酒花是多年生植物，其球果曾被用来酿造啤酒。这些啤酒花含有精油，主要是萜类化合物。常用的精油提取方法是庚烷溶剂萃取法或水蒸气蒸馏法，但这两种方法都会影响最终产品的质量。此外，所获得的提取物中的胡萝卜烯和月桂烯的数量和纯度仍然是一个挑战。此外，人们对石竹烯和法尼烯的提取知之甚少。本项目的主要目的是利用超临界CO2萃取技术，从Aratinga公司提供的级联酒花中提取胡萝卜烯、石竹烯、月桂烯和金合欢烯。以二氧化碳为溶剂的超临界萃取法可以很容易地将溶剂从提取的胡萝卜烯、石竹烯、月桂烯和金合欢烯中分离出来，留下一种易于表征的产品。在产率和产品质量方面，超临界萃取法的结果将与传统溶剂萃取法进行比较。高产量的纯胡萝卜烯、石竹烯、月桂烯和法尼烯将使它们进一步商业化，成为加拿大酿酒工业的原料，并将加强加拿大的农业和加工部门。

Hops Humulus lupulus are perennial plants, which cones have been used for beer making. These hops have essential oils, mainly terpenoids. Common extraction methods of essential oils are solvent extraction with heptane or steam distillation but both methods affect the quality of the final product. Also, the amount and purity of humulene, and myrcene in the extracts obtained are still a challenge. Furthermore, little is known on the extraction of caryophyllene, and farnesene. The main objective of this project is to use supercritical CO2 extraction, an environmentally-friendly "green" technology, to extract humulene, caryophyllene, myrcene and farnesene from Cascade hops provided by Aratinga Inc. Supercritical extraction using carbon dioxide as the solvent allows easy separation of the solvent from the extracted humulene, caryophyllene, myrcene and farnesene, leaving a product that is easy to characterize. Results from supercritical extraction in terms of yield and product quality will be compared to conventional solvent extraction. A high yield of pure humulene, caryophyllene, myrcene and farnesene will allow their further commercialization as ingredients to the brewery industry produced in Canada and will strengthen Canada's agricultural and processing sectors.