Innovative cryopreservation protocol optimization for bovine sperm and embryos.

牛精子和胚胎的创新冷冻保存方案优化。

• 批准号: 531082-2018
• 负责人: Benson, James
• 金额: $ 4.12万
• 依托单位: University of Saskatchewan
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=690682
• 关键词: Innovative; cryopreservation; protocol; optimization; bovine

This project seeks to optimize sperm and embryo cryopreservation protocols through novel mathematical modelling and experimental design. For bull sperm, we apply a novel strategy of iterative protocol and media optimization that capitalizes on existing damage and membrane transport models to inform next trial steps in the development of complete cryopreservation protocols. For bovine embryos, we will use mathematically guided real-time feedback control of embryo volumes via CPA concentrations to equilibrate embryos in high CPA concentrations with one of three mathematically optimal approaches: minimal time, minimal CPA toxicity, or minimal embryos volume excursion.

该项目旨在通过新颖的数学建模和实验设计来优化精子和胚胎冷冻保存方案。对于公牛精子，我们采用了一种新的迭代方案和介质优化策略，该策略利用现有的损伤和膜转运模型，以告知完整冷冻保存方案开发中的下一个试验步骤。对于牛胚胎，我们将通过CPA浓度使用数学指导的胚胎体积实时反馈控制，以三种数学最佳方法之一平衡高CPA浓度的胚胎：最短时间，最小CPA毒性或最小胚胎体积偏移。