Developing New Soft-Bonded Light-Reversible Polymer Bio-Materials

开发新型软粘合光可逆聚合物生物材料

• 批准号: RGPIN-2019-05661
• 负责人: Barrett, Christopher
• 金额: $ 5.76万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=692054
• 关键词: Developing; New; Soft; Bonded; Light

The long-term objective of this proposed research program is the development of new bio-inspired and bio-sourced polymer materials that are self-assembled non-covalently from aqueous solution to form stable bio-films and structures, and that also respond to mild stimuli (such as light) to effect local reversible structural transitions. An overarching goal lies in understanding how these photo-reversible bio-materials interact at real biological interfaces, and then exploring the extent of control one can gain over this interface for a variety of bio-medical or bio-engineering applications. These new polymer assemblies will mimic real biological tissue in their soft, wet, compliant tune-ability, and the light-responsive shape-changing azo dyes incorporated in small amounts mimic our rhodopsin/retinal systems that enable vision, to provide a direct opto-bio communication interface. From a practical standpoint these new polymers are of interest as smart' surfaces for signaling, sensing, and controlling adjacent biological activity, due to the combination of mild visible photochemical functionalities, and their inherent soft, wet bio-compatibility. From the standpoint of fundamental science, these new materials and their study ideally will enable us to contribute to basic understanding of biological function at the interface between living tissue/cells and artificial media, and communication between them, from a chemical and materials perspective. ******Built from water-soluble and bio-sourced components, when self-assembled together these new materials can form insoluble, stable, and strong polymeric materials. This controlled solubility transition to stability permits promising natural polymers to be incorporated for the first time (silk and other proteins, chitosan and other polysaccharides), to retain their desired properties yet prevent their ready solubility in water, and thus be applied as improved cell culture media, and eventually in vivo as biocompatible coatings for implants. This proposal represents the first expansion of our research from more simple artificial mimics of bio-polymers, into real bio-sourced superior natural polymers. This will enable us to develop entirely natural (ie: FDA-approved, even edible) polymeric assemblies for in vivo biomedical applications, that are completely soft-bonded together, to thus retain their biocompatibility and low toxicity. This could further allow soft-bound chemical cargo for delivery to be readily and gently loaded and released. The long-term vision of this research program lies in the development of new bio-inspired and bio-mimetic materials that can be self-assembled from aqueous solution and respond to light, understanding how they interact with real biological interfaces, and then exploring the extent of control over this interface for biomedical applications, now using nature as the inspiration for both the soft organic materials, and for light as the communication medium.

该提出的研究计划的长期目标是开发新的生物启发和生物产生的聚合物材料，这些聚合物材料是从水溶液中非共价组装而成的，以形成稳定的生物膜和结构，并对轻度刺激（例如光）反应以实现局部可逆的结构过渡。 总体目标在于了解这些光可逆的生物材料如何在实际生物界面上相互作用，然后探索控制的程度，可以在此界面上获得各种生物医学或生物工程应用程序。 这些新的聚合物组件将模仿其柔软，潮湿，兼容的曲调能力中的真实生物组织，以及少量掺入的变化形状的变形的偶氮染料模仿我们的视hopopsin/视网膜系统，以使视觉能够提供视觉，以提供直接的Opto-Bio通信界面。 从实际的角度来看，由于轻度可见的光化学功能及其固有的柔软，潮湿的生物兼容性，这些新聚合物作为信号传导，传感和控制相邻生物学活动的智能表面。 从基本科学的角度来看，这些新材料及其研究理想地将使我们能够从化学和材料的角度从生物组织/细胞和人工培养基之间的界面以及它们之间的交流来做出基本了解。 ******由水溶性和生物产生的组件建造，当自组装在一起时，这些新材料会形成不溶性，稳定且强大的聚合物材料。 这种受控的溶解度过渡到稳定性允许首次掺入有希望的天然聚合物（丝绸和其他蛋白质，壳聚糖和其他多聚糖），保留其所需的特性，但可以防止其现成的溶解度，从而在水中溶解，从而将其应用于改善的细胞培养基，并最终以生物相称的涂料为生涂料。该提案代表了我们研究的首次扩展，从更简单的生物聚合物的模仿到真正的生物产生的高级天然聚合物。 这将使我们能够为体内生物医学应用完全自然（即：FDA批准的，甚至是可食用的）聚合物组件，它们完全粘合在一起，从而保持其生物相容性和低毒性。这可能会进一步使软化的化学货物容易被轻轻加载和释放。该研究计划的长期愿景在于开发新的生物启发和生物模拟材料，这些材料可以从水溶液中自我组装，对光线进行反应，了解它们如何与真实的生物学接口相互作用，然后探索对生物医学应用程序的控制程度，现在以生物医学应用程序的方式来吸引这种界面，从而使大自然作为柔软的有机材料的启发，并作为柔软的有机材料，并用作光线介质。