A Platform for Assessment of Samples from Biomolecules to Living Cells

在微孔板上对活细胞的光学、化学或生物信号进行高通量、实时检测是一种创新的方法，可以有效地研究各种条件下最自然状态下的细胞事件。这项尖端技术彻底改变了开发基于细胞的检测方法的能力，以回答生命科学中的基本问题，如细胞分化和免疫反应。所要求的多模式平板阅读器是一个平台，不仅可以测量生物分子样品（如酶、抗体、核酸）的紫外/可见吸光度、荧光和发光，还可以以亚秒级的时间分辨率对活细胞、三维球体进行成像。该平板阅读器对于实施生物分子和细胞系统的复杂实验至关重要，包括酶分析，表型分析，分子靶向，细胞增殖和细胞毒性分析，生物标志物定量，以及培养下一代充满激情的科学家。******车牌阅读器是由nserc资助的10名研究人员组成的团队要求的，其中包括5名从事生物化学和生命科学研究的早期职业调查员。该团队非常成功，出版了具有国际影响力的出版物，仅在过去三年里，就从三理事会机构获得了300多万美元的运营资助。然而，缺乏最先进的、能够成像的多模式车牌读取器阻碍了该团队的研究进展。获取的延迟严重影响了这些实验室的HQP培训，并可能对酶学、细胞生物学、神经科学、免疫学和组织生理学等领域的研究项目产生长期的负面影响。******所要求的仪器是整个温莎-埃塞克斯地区快速增长的生物化学和生命科学研究界的关键工具。生命科学是温莎大学的战略重点之一，温莎大学坚定地承诺建造一座新的研究大楼（埃塞克斯研究中心），在化学与生物化学和生物医学科学系战略性地雇用了五名新教员，并提供了35,000美元的研究刺激基金，以补充本申请所要求的资金。所要求的多模式平板阅读器将弥补研究能力的重大不足，并将促进世界一流的生物化学和生命科学研究项目。******该平台将立即为两个部门的十个研究项目（三个申请人和七个经常用户）提供支持。该设备仅在第一年就对至少50名HQP的培训至关重要。值得注意的是，所申请的仪器对若干合作、多学科团队资助的进展至关重要，包括药物发现、蛋白质组学、生物技术、细胞生物学等领域的国家和国际伙伴关系。********

High-throughput, real-time detection of optical, chemical, or biological signals from living cells in a microplate is an innovative approach to efficiently study cellular events under various conditions in their most natural state. This cutting-edge technology revolutionized the ability to develop cell-based assays to answer fundamental questions in life sciences, such as cell differentiation and immune response. The requested multi-mode plate reader is a platform that not only measures UV/visible absorbance, fluorescence, and luminescence of biomolecular samples, such as enzymes, antibodies, nucleic acids, but also images living cells, three-dimensional spheroids at sub-second temporal resolution. This plate reader is absolutely vital for implementing complex experiments on biomolecular and cellular systems, including enzymatic assays, phenotypic assays, molecular targeting, cell proliferation and cytotoxicity analysis, biomarker quantification, and for training the next generation of impassioned scientists.******The plate reader is requested by a team of ten NSERC-funded researchers, including five early-career investigators, engaged in biochemistry and life science studies. The team is highly successful with publications of international impact and more than $3M operating grants from the tri-council agencies alone in the last three years. However, the lack of a state-of-the-art, multi-mode plate reader capable of imaging hampers the team's research progress. Delay in acquisition has severe implications for the training of HQP from these laboratories and has the potential of a long-lasting negative impact on research programs spanning the fields of enzymology, cell biology, neuroscience, immunology, and tissue physiology.******The requested instrument is a critical tool for a rapidly growing research community of biochemistry and life sciences across the Windsor-Essex region. Life sciences is one of the strategic priorities at the University of Windsor demonstrated by a strong commitment with a new research building (Essex Centre of Research), the strategic hiring of five new faculty members in the departments of Chemistry & Biochemistry and Biomedical Sciences, and the provision of a research stimulus fund of $35,000 to supplement the requested fund of this application. The requested multi-mode plate reader will fulfill a significant deficiency in the research capability and will promote world-class research programs in biochemistry and life sciences.******This platform will immediately provide support to ten research programs (three applicants and seven frequent users) across two departments. This equipment is critical for the training of at least 50 HQP in the first year alone. Important to note, the requested instrument is central to the progress of several collaborative, multi-disciplinary team grants that include national and international partnerships in the fields of drug discovery, proteomics, biotechnology, cellular biology, and many others.********