Strain engineering and bioprocessing strategies for bio-based production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) using cheap feedstocks

使用廉价原料生物生产聚（3-羟基丁酸酯-co-3-羟基戊酸酯）（PHBV）的菌株工程和生物加工策略

• 批准号: 539590-2019
• 负责人: Chou, CPerry
• 金额: $ 10.96万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=693847
• 关键词: Strain; engineering; bioprocessing; strategies; bio

Due to unstable supply of fossil fuels, numerous environmental concerns of climate change, stringent regulations for petro-based production, and motivation to "low carbon" economy, replacing petrochemical processes with biomass-biological strategies has been recognized as a modern technology for renewable, sustainable, and clean manufacturing of value-added chemicals and fuels. Polyhydroxyalkanoates (PHAs) are natural polyesters recently attracting considerable interests as promising candidates to replace petro-based synthetic plastics with numerous environmental impacts. Based on common interests in bio-based production of PHAs, our biomanufacturing research group at UWaterloo and Genecis Bioindustries, a Toronto-based company, initiate the proposed research with a major objective to develop an advanced whole-cell transformation platform, including genetically and metabolically engineered bacterial strains, and their associated bioprocessing strategies, for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), a biocopolymer member of PHAs, using cheap feedstocks, such as glycerol and volatile fatty acids (VFAs). Genecis has recently decided to explore PHBV-related technologies upon recognizing certain limitations associated with their existing PHA bioprocess and products. The developed engineered bacterial strains and bioprocesses for PHBV production can be readily transferred to Genecis for large-scale production, benefiting the existing Canadian biopolymer industry not only technologically but also economically. The proposed research is also highly multidisciplinary in biological sciences and industrial biotechnology with the following envisioned outcomes and benefits: (1) developing a clean, renewable, sustainable, and economically feasible PHBV bioprocess; (2) boosting Canada's research capacity and technological development in bioprocessing strategies and biopolymer production; (3) improving Canada's living environment and mitigating greenhouse gas emissions through clean biomanufacturing; and (4) training highly qualified personnel (HQP) for meeting challenges of the next generation of industrial biotechnology.

由于化石燃料供应不稳定、气候变化带来的诸多环境问题、对石油生产的严格监管以及“低碳”经济的动机，用生物质-生物战略取代石化过程已被公认为可再生、可持续和清洁制造增值化学品和燃料的现代技术。聚羟基烷酸酯（PHAs）是一种天然聚酯，近年来作为有希望取代石油基合成塑料的候选材料引起了人们的广泛关注。基于对pha生物基生产的共同兴趣，我们在UWaterloo的生物制造研究小组和总部位于多伦多的Genecis Bioindustries公司发起了这项研究，其主要目标是开发一种先进的全细胞转化平台，包括遗传和代谢工程细菌菌株，及其相关的生物处理策略，用于生产pha的生物聚合物成员聚（3-羟基丁酸酯-co-3-羟基戊酸酯）（PHBV）。使用廉价的原料，如甘油和挥发性脂肪酸（VFAs）。在认识到现有PHA生物工艺和产品的某些局限性后，Genecis最近决定探索与phbv相关的技术。开发的用于PHBV生产的工程菌株和生物工艺可以很容易地转移到Genecis进行大规模生产，这不仅在技术上而且在经济上有利于现有的加拿大生物聚合物工业。拟议的研究在生物科学和工业生物技术方面也具有高度的多学科性，其预期结果和效益如下：(1)开发清洁、可再生、可持续和经济上可行的PHBV生物工艺；(2)促进加拿大在生物加工策略和生物聚合物生产方面的研究能力和技术发展；(3)通过清洁生物制造改善加拿大的生活环境，减少温室气体排放；(4)培养高素质人才，迎接下一代工业生物技术的挑战。