Contact killing of COVID-19 by copper-based nanoparticles.

铜基纳米粒子接触杀死 COVID-19。

• 批准号: 554545-2020
• 负责人: Labbe, Simon
• 金额: $ 3.64万
• 依托单位: Université de Sherbrooke
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=704789
• 关键词: Contact; killing; COVID; 19; copper

The primary goal of this proposal is to decipher the virucidal efficacy of carboxymethyl cellulose copper (CMC-Cu) nanoparticles against COVID-19 (formally named SARS-CoV-2). The studies will be performed in partnership with the Canadian company Sani Marc Group. Microbes are rapidly killed on surfaces of copper and copper alloys through a process named copper-mediated "contact killing". Furthermore, a recent study has revealed that COVID-19 is not viable upon exposure to copper surfaces. Of significance, copper concentrations required to kill microbes are not toxic for humans. Creating "self-disinfecting surfaces" by coating inanimate surfaces with copper is difficult to apply for practical and economic reasons. However, an exciting avenue is the recent discovery of CMC-Cu as an effective alternative to one-step disinfectant agent that significantly reduces microbial levels on surfaces and inanimate objects. Using an innovative method enabling production of MLV-based COVID-19 pseudotyped particles in a biosafety level 2 laboratory, we will investigate COVID-19 stability and its ability to attach and infect target cells in the presence of CMC-Cu in the absence of other reagent or in the presence of selected Sani Marc's disinfectant products. During the next year, we will focus our research program on the following specific aims. 1. Production of MLV-based COVID-19 Spike-pseudotyped particles containing a reporter gene that enables quantification of virus entry into host cells. 2. Mode of action of CMC-Cu-mediated inactivation of COVID-19 Spike-pseudotyped particles. 3. Determination of how are COVID-19 Spike-pseudotyped particles killed in the presence of CMC-Cu. Students trained under this program will acquire education/training in multidisciplinary approaches, including areas of nanotechnology, virology, biochemistry, molecular and cellular biology. Students without distinction as to minorities will develop critical skills, allowing them to gain the status of highly trained scientists for the Canadian biotech sector and/or academic research community.

该提案的主要目标是破译羧甲基纤维素铜（CMC-Cu）纳米颗粒对COVID-19（正式名称为SARS-CoV-2）的杀病毒功效。这些研究将与加拿大公司Sani Marc Group合作进行。铜和铜合金表面上的微生物通过一种称为铜介导的“接触杀灭”的过程被迅速杀灭。此外，最近的一项研究表明，COVID-19在暴露于铜表面时无法存活。重要的是，杀死微生物所需的铜浓度对人类无毒。由于实际和经济的原因，通过用铜涂覆无生命表面来创建“自消毒表面”是难以应用的。然而，一个令人兴奋的途径是最近发现CMC-Cu作为一步消毒剂的有效替代品，可显著降低表面和无生命物体上的微生物水平。我们将使用一种创新的方法，在生物安全2级实验室中生产基于MLV的COVID-19假型颗粒，研究COVID-19的稳定性及其在CMC-Cu存在下在不存在其他试剂或存在选定的Sani Marc消毒剂产品的情况下附着和感染靶细胞的能力。 在接下来的一年里，我们将把研究计划集中在以下具体目标上。 1.生产基于MLV的COVID-19刺突假型颗粒，其含有能够定量病毒进入宿主细胞的报告基因。 2. CMC-Cu介导的COVID-19刺突假型颗粒灭活的作用方式。 3.确定CMC-Cu存在下COVID-19刺突假型颗粒如何被杀死。 根据该计划培训的学生将获得多学科方法的教育/培训，包括纳米技术，病毒学，生物化学，分子和细胞生物学领域。学生不分少数民族将发展关键技能，使他们能够获得加拿大生物技术部门和/或学术研究界训练有素的科学家的地位。