Market assessment of water-soluble chromogenic substrates for detecting bacterial beta-lactamases

用于检测细菌β-内酰胺酶的水溶性显色底物的市场评估

• 批准号: 560497-2021
• 负责人: Dmitrienko, Gary
• 金额: $ 1.09万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Idea to Innovation
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=707127
• 关键词: Market; assessment; water; soluble; chromogenic

Beta-lactam antibiotics such as penicillins and cephalosporins are the most widely prescribed pharmaceuticals for treating potentially life-threatening bacterial infections. Human pathogenic bacteria are becoming increasingly resistant to antibiotics in general. In the case of the beta-lactam antibiotics, the main cause of antibiotic resistance is the ability of bacteria to produce enzymes called beta-lactamases that catalyze a chemical reaction of the beta-lactam with water resulting in the destruction of the ability of these pharmaceuticals to act as antibiotics. Recent research at the University of Waterloo has led to the discovery of a synthetic compound, now called Aubergen2, that undergoes a very dramatic (chromogenic) colour change from yellow to deep purple in the presence of bacterial beta-lactamases thus allowing for a very rapid visual method for detecting the presence of bacteria that are resistant to beta-lactam antibiotics. Aubergen2 has the potential to provide substantial improvement in the area of visual detection of antibiotic resistance to guide the choice of the most appropriate antibiotics for treatment of bacterial infections. Additionally, this chromogenic compound has much potential for use in the discovery of inhibitors of the bacterial beta-lactamases that can be administered along with beta-lactam antibiotics to overcome antibacterial resistance as well as in new biotechnological methods in areas as diverse as virus detection to detection of asbestos. This proposal seeks to enlist an independent group to carry out a market analysis to determine if this technology has the potential to be competitive in the Canadian and world-wide markets for chromogenic enzyme substrates.

β-内酰胺类抗生素如青霉素和头孢菌素是治疗潜在危及生命的细菌感染的最广泛的处方药。 人类病原菌对抗生素的耐药性越来越强。 在β-内酰胺抗生素的情况下，抗生素耐药性的主要原因是细菌产生称为β-内酰胺酶的酶的能力，该酶催化β-内酰胺与水的化学反应，导致这些药物作为抗生素的能力被破坏。 滑铁卢大学最近的研究发现了一种合成化合物，现在称为Aubergen 2，在细菌β-内酰胺酶的存在下，它会发生非常戏剧性的（显色）颜色变化，从黄色到深紫色，从而允许一种非常快速的视觉方法来检测对β-内酰胺抗生素耐药的细菌的存在。 Aubergen 2有可能在抗生素耐药性的视觉检测领域提供实质性的改进，以指导选择最合适的抗生素治疗细菌感染。 此外，该显色化合物在发现细菌β-内酰胺酶抑制剂方面具有很大的潜力，所述抑制剂可以与β-内酰胺抗生素一起沿着施用以克服抗菌剂耐药性，以及在从病毒检测到石棉检测等不同领域的新生物技术方法中具有很大的潜力。 该提案旨在招募一个独立的小组进行市场分析，以确定该技术是否有潜力在加拿大和世界范围的显色酶底物市场上具有竞争力。