Probing of cortical-hippocampal dialogue using light-mediated circuit monitoring in vivo

使用体内光介导电路监测探测皮质-海马对话

• 批准号: RGPIN-2015-05535
• 负责人: Mohajerani, Majid
• 金额: $ 2.77万
• 依托单位: University of Lethbridge
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=707946
• 关键词: Probing; cortical; hippocampal; dialogue; using

We spend about one third of our lives sleeping. Perhaps surprisingly, our brain never rests and neurons remain continually active during sleep. One can reason that neurons responsible for autonomic functions such as respiration should be active in both sleep and wakefulness, however, why would neurons in other parts of the brain remain active, often in a highly synchronous and rhythmic fashion during sleep? One proposed idea is that neural activity during sleep may allow for the rewiring of neural connections and that this synaptic rewiring is relevant for storing memories. My focus is on understanding memories that require medial temporal lobe structures like the hippocampus (eg. episodic memory) and the memory-enhancing role of particular patterns of spontaneous activity that appear during sleep in neocortex (NC) and hippocampus (HPC). The central goal of my research is to provide new information regarding the interaction of HPC and NC during sleep. Although evidence indicates that these memories are formed through a functional coupling between hippocampal sharp-wave (SPW) and cortical slow-wave oscillations (SO), how they interact to subserve a transfer of information is still unclear. To provide new insight regarding the interaction of HPC and NC during sleep, we will combine novel imaging technologies with the most advanced genetic, molecular and electrophysiological technologies. This will allow us to interrogate the cortical circuits at the level of individual synapses and function with millisecond temporal resolution across the entire cortex with information from the hippocampus. The primary aim of my research will be to examine how SO and SPW-, and other events (i.e. spindles, gamma oscillations) are embedded in larger cortical networks. By selectively and temporarily inactivating SPW, I will test a causal link between HPC activity and NC distinct network activity patterns. Further, I will dissect how HPC output to NC contributes significantly to experience-dependent dendritic spine turnover and plasticity. Performing these experiments will provide a deeper understanding of the functional relationship between hippocampus and neocortex and information processing and long-term memory storage.

我们一生中大约有三分之一的时间是在睡觉。也许令人惊讶的是，我们的大脑从不休息，神经元在睡眠期间保持持续活跃。人们可以推断，负责呼吸等自主神经功能的神经元在睡眠和清醒时都应该是活跃的，然而，为什么大脑其他部分的神经元仍然活跃，通常在睡眠期间以高度同步和有节奏的方式保持活跃？一种提出的想法是，睡眠期间的神经活动可能允许神经连接的重新连接，这种突触重新连接与存储记忆有关。我的重点是理解需要内侧颞叶结构的记忆，如海马体(例如。情景记忆)，以及睡眠期间出现的特定自发活动模式在新皮质(NC)和海马体(HPC)中的增强记忆作用。我的研究的中心目标是提供关于睡眠期间HPC和NC相互作用的新信息。尽管有证据表明，这些记忆是通过海马体尖波(SPW)和皮层慢波振荡(SO)之间的功能耦合形成的，但它们如何相互作用以辅助信息传递仍不清楚。为了提供关于睡眠期间HPC和NC相互作用的新见解，我们将把新的成像技术与最先进的遗传、分子和电生理技术相结合。这将使我们能够在单个突触的水平上询问皮质回路，并利用来自海马体的信息以毫秒级的时间分辨率在整个皮质发挥作用。我的研究的主要目的将是研究SO和SPW-以及其他事件(即纺锤体、伽马振荡)是如何嵌入更大的皮质网络的。通过有选择地暂时停用SPW，我将测试HPC活动和NC不同网络活动模式之间的因果联系。此外，我将剖析高性能混凝土对NC的输出如何显著有助于经验依赖型树突棘的周转和可塑性。进行这些实验将更深入地了解海马体和新皮质之间的功能关系，以及信息处理和长期记忆存储。