Plant microbiome manipulation: nitrogen as the key currency
植物微生物组调控:氮作为关键货币
基本信息
- 批准号:RGPIN-2020-05723
- 负责人:
- 金额:$ 3.06万
- 依托单位:
- 依托单位国家:加拿大
- 项目类别:Discovery Grants Program - Individual
- 财政年份:2020
- 资助国家:加拿大
- 起止时间:2020-01-01 至 2021-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Nitrogen (N) availability is the main factor limiting growth in terrestrial ecosystems. Indiscriminate use of nitrogen fertilization was central to the yield boost brought by the Green Revolution. However, N fertilization is now at the forefront of the environmental and economical issues facing agriculture. In view of its limiting nature, I hypothesize that competition for N is a central mechanism regulating plant-microbe interactions at the soil-root interface. I have gathered preliminary data that show that the plant intendedly inhibits the amino acid permease gene expression of bacteria through the secretion of micro RNAs (miRNAs) in its rhizosphere. This would result in a larger part of the soil amino acid pool being routed to the plant. Additionally, I have shown that ammonia-oxidizers have a key role in determining wheat yields and grain quality, much more than the amount of applied fertilizer. Ammonia oxidizers are bacteria and archaea that transform ammonia to nitrate in order to generate energy that they use to fix carbon dioxide (chemosynthesis). As such, they are completely independent from the carbon provided by the plant, but they need ammonia from fertilizer or from the degradation of organic nitrogen to fulfill their energy requirement. At the same time, ammonia is energetically more favorable for plant growth than nitrate, putting the needs of the plant and of the ammonia-oxidizers head-to-head. My research program aims at 1) understanding and demonstrating the key role of nitrogen in plant-microbe interactions, both for organic and inorganic nitrogen sources, and 2) finding novel ways to modify plant-microbe interactions using nitrogen. More specifically, my short-term objectives are to 1) Determine the role of plant miRNAs in the competition for N in the rhizosphere, 2) Determine the role of amino acids in the competition for N in the rhizosphere and 3) Manipulate the plant microbiome by changing the competition for N. My research program will provide an advanced understanding of the rules governing plant-microbe interactions, leading to novel approaches to shape the plant microbiota toward an optimization of crop yields and quality. The combination of innovative experiments combined with advanced molecular methods such as sequencing, bioinformatics and multivariate statistics will prepare the 3 PhD students, 3 MSc students and 10 undergraduate students involved in my program to take up central positions in the industry, the government or academia.
氮的有效性是限制陆地生态系统生长的主要因素。不加区别地使用氮肥是绿色革命带来的产量提高的核心。然而,氮肥施肥现在是农业面临的环境和经济问题的前沿。鉴于其限制性的性质,我假设N的竞争是调节植物-微生物在土壤-根界面的相互作用的一个中心机制。我已经收集了初步的数据,表明植物通过在其根际分泌微小RNA(miRNAs)有意抑制细菌的氨基酸通透酶基因表达。这将导致土壤氨基酸库的大部分被路由到植物。此外,我已经表明,氨氧化剂在决定小麦产量和谷物品质方面发挥着关键作用,远远超过施肥量。氨氧化菌是将氨转化为硝酸盐的细菌和古细菌,以产生用于固定二氧化碳的能量(化学合成)。因此,它们完全独立于植物提供的碳,但它们需要来自肥料或有机氮降解的氨来满足其能量需求。与此同时,氨在能量上比硝酸盐更有利于植物生长,这使得植物和氨氧化剂的需求直接相关。我的研究项目旨在1)了解和证明氮在植物与微生物相互作用中的关键作用,无论是有机氮源还是无机氮源,以及2)寻找使用氮改变植物与微生物相互作用的新方法。更具体地说,我的短期目标是1)确定植物miRNA在根际氮竞争中的作用,2)确定氨基酸在根际氮竞争中的作用,3)通过改变氮竞争来操纵植物微生物组。我的研究计划将提供对植物-微生物相互作用规则的深入了解,从而找到新的方法来塑造植物微生物群,以优化作物产量和质量。创新实验与先进的分子方法相结合,如测序,生物信息学和多元统计学,将为参与我的计划的3名博士生,3名硕士生和10名本科生做好准备,以便在行业,政府或学术界担任核心职位。
项目成果
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Yergeau, Etienne其他文献
Early season soil microbiome best predicts wheat grain quality
- DOI:
10.1093/femsec/fiac144 - 发表时间:
2022-12-14 - 期刊:
- 影响因子:4.2
- 作者:
Asad, Numan Ibne;Wang, Xiao-Bo;Yergeau, Etienne - 通讯作者:
Yergeau, Etienne
Predictable bacterial composition and hydrocarbon degradation in Arctic soils following diesel and nutrient disturbance
- DOI:
10.1038/ismej.2013.1 - 发表时间:
2013-06-01 - 期刊:
- 影响因子:11
- 作者:
Bell, Terrence H.;Yergeau, Etienne;Greer, Charles W. - 通讯作者:
Greer, Charles W.
Impact of water quality on the bacterial populations and off-flavours in recirculating aquaculture systems
- DOI:
10.1111/1574-6941.12053 - 发表时间:
2013-05-01 - 期刊:
- 影响因子:4.2
- 作者:
Auffret, Marc;Yergeau, Etienne;Villemur, Richard - 通讯作者:
Villemur, Richard
Shifts in soil microorganisms in response to warming are consistent across a range of Antarctic environments
- DOI:
10.1038/ismej.2011.124 - 发表时间:
2012-03-01 - 期刊:
- 影响因子:11
- 作者:
Yergeau, Etienne;Bokhorst, Stef;Kowalchuk, George A. - 通讯作者:
Kowalchuk, George A.
Soil Characteristics Constrain the Response of Microbial Communities and Associated Hydrocarbon Degradation Genes during Phytoremediation
- DOI:
10.1128/aem.02170-20 - 发表时间:
2021-01-01 - 期刊:
- 影响因子:4.4
- 作者:
Correa-Garcia, Sara;Rheault, Karelle;Yergeau, Etienne - 通讯作者:
Yergeau, Etienne
Yergeau, Etienne的其他文献
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{{ truncateString('Yergeau, Etienne', 18)}}的其他基金
Plant microbiome manipulation: nitrogen as the key currency
植物微生物组调控:氮作为关键货币
- 批准号:
RGPIN-2020-05723 - 财政年份:2022
- 资助金额:
$ 3.06万 - 项目类别:
Discovery Grants Program - Individual
Plant microbiome manipulation: nitrogen as the key currency
植物微生物组调控:氮作为关键货币
- 批准号:
RGPIN-2020-05723 - 财政年份:2021
- 资助金额:
$ 3.06万 - 项目类别:
Discovery Grants Program - Individual
Plant Microbiome Engineering
植物微生物组工程
- 批准号:
RGPIN-2014-05274 - 财政年份:2019
- 资助金额:
$ 3.06万 - 项目类别:
Discovery Grants Program - Individual
Novel holobiont-based approaches to rapidly adapt wheat to drought stress
基于全生物的新方法使小麦快速适应干旱胁迫
- 批准号:
494702-2016 - 财政年份:2018
- 资助金额:
$ 3.06万 - 项目类别:
Strategic Projects - Group
Plant Microbiome Engineering
植物微生物组工程
- 批准号:
RGPIN-2014-05274 - 财政年份:2018
- 资助金额:
$ 3.06万 - 项目类别:
Discovery Grants Program - Individual
Novel holobiont-based approaches to rapidly adapt wheat to drought stress
基于全生物的新方法使小麦快速适应干旱胁迫
- 批准号:
494702-2016 - 财政年份:2017
- 资助金额:
$ 3.06万 - 项目类别:
Strategic Projects - Group
Identification d'indicateurs microbiologiques du sol liés à la qualité de la farine
太阳微生物微生物指标鉴定
- 批准号:
514682-2017 - 财政年份:2017
- 资助金额:
$ 3.06万 - 项目类别:
Engage Grants Program
Plant Microbiome Engineering
植物微生物组工程
- 批准号:
RGPIN-2014-05274 - 财政年份:2017
- 资助金额:
$ 3.06万 - 项目类别:
Discovery Grants Program - Individual
Plant Microbiome Engineering
植物微生物组工程
- 批准号:
RGPIN-2014-05274 - 财政年份:2016
- 资助金额:
$ 3.06万 - 项目类别:
Discovery Grants Program - Individual
Plant Microbiome Engineering
植物微生物组工程
- 批准号:
RGPIN-2014-05274 - 财政年份:2015
- 资助金额:
$ 3.06万 - 项目类别:
Discovery Grants Program - Individual
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