Understanding the Biochemical Characteristics of Canine Saliva

了解犬唾液的生化特征

• 批准号: RGPIN-2020-06119
• 负责人: Siqueira, Walter
• 金额: $ 2.4万
• 依托单位: University of Saskatchewan
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=750697
• 关键词: Understanding; Biochemical; Characteristics; Canine; Saliva

Proteomics studies have been initiated in veterinary science; specifically, the proteome exploration in cells, secretions, and tissues of farm animals has increased exponentially. Still, the proteome of companion animals, like dogs, has not been studied in detail. Despite the weight of saliva in the regulation of oral homeostasis in most vertebrates, insufficient studies have been conducted to investigate the salivary composition of different mammal species. Dog saliva is very poorly studied in terms of composition, physiology, and biochemistry. Based on the little that is known about the salivary composition present in the dog mouth, we can highlight significant differences between human and dog saliva. The lack of attention to such critical variations between human and dog saliva and the gap of foundational studies in dog saliva may partially explain why the use of therapeutics developed based on human saliva are not efficient in dogs. Among the diseases that affect dogs, dental calculus and periodontitis are common. In nature, the main protection against these diseases are the salivary components Hypothesis: Dog's saliva has unique composition and biochemistry characteristics that are responsible for promoting oral cavity homeostasis My short-term goals are to understand the dog saliva biochemistry. Here, this grant includes 3 novel areas of investigation: Aim 1: Investigation of the proteome and peptidome in dog saliva Aim 2: Identification of phosphoproteome and their phosphorylated sites in dog saliva Aim 3: Determination of salivary flow rate, inorganic composition, pH and buffer capacity in dog saliva Research Plan: Saliva from dogs will be collected. In addition, saliva will be subjected to proteomic and peptidomic analysis. All acquired mass spectrum data will be analyzed against a comprehensive dog, mammalian and human protein databank (Aim 1). Finally, a phosphoproteome approach will be used to identify the phosphoproteins and their phosphorylation sites (Aim 2). Also, samples will be analyzed for ion composition, pH, and buffer capacity using analytical methods(Aim 3) Relevance of the study: I am proposing the first 5 years of a pioneering scientific exploration towards a comprehensive characterization of the dog saliva. For the first time, I plan to characterize the peptidome and phosphoproteome of dog saliva. As a long-term goal, I will use the research findings generated from this application for continuing my research program where I plan to understand the formation of dental calculus and periodontal disease in dogs. Moreover, I believe that the overall outcome of such OMICS studies has the potential to open novel avenues of investigations with direct relevance to veterinary sciences by identifying biomarkers for dogs oral and systemic diseases - a research area with significant appeal with the Pet Private-Sector where I plan to engage by using a more translational grant tool like NSERC-ALLIANCE grant and CHRP grant

蛋白质组学研究始于兽医科学；具体地说，农场动物细胞、分泌物和组织中的蛋白质组研究呈指数级增长。然而，狗等伴生动物的蛋白质组还没有得到详细研究。尽管在大多数脊椎动物中，唾液在调节口腔动态平衡方面起着举足轻重的作用，但对不同哺乳动物物种的唾液组成的研究还不够深入。狗的唾液在成分、生理和生物化学方面的研究非常少。基于对狗口腔中唾液成分知之甚少的情况，我们可以强调人和狗唾液之间的显著差异。缺乏对人和狗唾液之间这种关键差异的关注，以及狗唾液基础研究的空白，可能在一定程度上解释了为什么基于人类唾液开发的疗法在狗身上无效。在影响狗的疾病中，牙结石和牙周炎是常见的。在自然界中，对这些疾病的主要预防是唾液成分假说：狗的唾液具有独特的组成和生化特性，这些特性有助于促进口腔内环境的稳定我的短期目标是了解狗的唾液生化。在这里，这笔赠款包括三个新的研究领域：目标1：调查狗唾液中的蛋白质组和肽组。目标2：鉴定狗唾液中的磷酸蛋白质组及其磷酸化位置。目标3：测定狗唾液中的唾液流速、无机成分、pH和缓冲能力。研究计划：收集狗的唾液。此外，还将对唾液进行蛋白质组学和多肽分析。将根据一个全面的狗、哺乳动物和人类蛋白质数据库(目标1)对所有获得的质谱学数据进行分析。最后，将使用磷化蛋白质组方法来鉴定磷化蛋白及其磷酸化位点(目标2)。此外，还将使用分析方法分析样本的离子组成、pH和缓冲容量(目标3)研究的相关性：我提议进行一项开创性的科学探索的头5年，以全面表征狗的唾液。第一次，我计划描述狗唾液中的多肽组和磷蛋白质组。作为一个长期目标，我将利用这项申请产生的研究成果继续我的研究计划，在那里我计划了解狗的牙结石和牙周病的形成。此外，我相信OMICS研究的总体结果有可能开辟与兽医科学直接相关的新的调查途径，通过识别狗的口腔和全身疾病的生物标记物-这是一个对宠物私营部门具有重大吸引力的研究领域-我计划通过使用更具翻译性的赠款工具，如NSERC-联盟补助金和CHRP补助金，参与其中