Thermal Desorption Unit for Gas Chromatographic Mass Spectrometric Analyses

用于气相色谱质谱分析的热脱附装置

• 批准号: RTI-2021-00382
• 负责人: Hillier, Neil
• 金额: $ 6.99万
• 依托单位: Acadia University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=718549
• 关键词: Thermal; Desorption; Unit; Gas; Chromatographic

One of the Hillier lab's primary foci is the investigation of alternative management strategies using chemical ecology of insect pheromones and/or volatile emissions from plants. To facilitate this need, we use gas-chromatography to identify and characterize useful naturally-occurring chemical compounds for use in insect pest management. By characterizing such compounds, we can then examine the resultant behavioural and neurophysiological consequences, and develop new tools for insect pest management. Funds are requested to purchase a thermal desorption unit (TDU) to expand utilization of current equipment in our laboratory. Thermal desorption involves directly heating a material (e.g. insect, plant, soil) to release adsorbed compounds from it and directly injects the material into a GCMS (Gas Chromatograph-Mass Spectrometer) for identification and analysis. Furthermore, this allows for selective collection of volatiles and concentration of analytes with specific sorbents, thermal ranges, and gas flow. Since this technique does not require solvents, it also minimizes contamination or loss via dilution. Finally, this technique has recently become very practical for analysis of volatiles from small invertebrates, as living samples may be inserted directly into analytical tubes and heated for direct analyte collection. This proposal requests an upgrade of this device, which will be essential to expanding my research program (and others) to encompass new experimental protocols, faster throughput on analyses, and the creation of a landmark facility for training students and professionals. By enhancing the capabilities of this infrastructure, it is anticipated that this technology will permit multiple collaborations within the agricultural and forestry sectors, having potential to expand this research in Atlantic Canada.

Hillier实验室的主要重点之一是利用昆虫信息素和/或植物挥发性排放物的化学生态学来研究替代管理策略。为了满足这一需求，我们使用气相色谱法来识别和表征用于害虫管理的有用的天然化合物。通过表征这些化合物，我们可以检查由此产生的行为和神经生理后果，并开发害虫管理的新工具。