Peptide synthesizer essential for natural products and chemical biology research

我们迫切需要一个替代的固相肽合成器。在我们的研究小组中，获得肽，包括那些被非蛋白质原性氨基酸取代的肽，对于HQP来说是必不可少的，可以表征肽的天然产物生物合成途径，研究dna结合碳氢化合物钉接肽，开发标记蛋白质的探针，以及研究生物环境中的酶。我们之前的固相肽合成器，一种CEM微波肽合成器，已经被制造商淘汰，替换零件和消耗品都不再可用，导致仪器的功能即将丧失，从而对所有HQPs的培训和进展产生负面影响。校园里没有任何仪器可以取代这一重要的研究工具。如果没有这些基础设施，body实验室将无法维持其国际公认的前沿研究项目，即利用天然产物生物合成来获取复杂分子，Keillor实验室也将无法保持其在化学工具开发方面的世界领先地位，以研究谷氨酰胺转酶（TGase）生物学。

We desperately require a replacement solid phase peptide synthesizer. Access to peptides, including those substituted with non-proteinogenic amino acids, is essential for HQP in our research groups to characterize peptidic natural product biosynthetic pathways, study DNA-binding hydrocarbon stapled peptides, develop probes for labeling proteins, and to study enzymes in biological settings. Our previous solid phase peptide synthesizer, a CEM microwave peptide synthesizer, has been phased out by the manufacturer and both replacement and consumable parts are no longer available, leading to imminent loss of function for the instrument, thereby negatively impacting the training and progress through their degrees for all HQPs. No instrument is available on campus to replace this essential research tool. Without this infrastructure, the Boddy lab will not be able to maintain its internationally recognized, cutting edge research program on harnessing natural product biosynthesis to access complex molecules, nor will the Keillor lab be able to retain its position as a world leader in the development of chemical tools to interrogate transglutaminase (TGase) biology.