Peptide synthesizer essential for natural products and chemical biology research

天然产物和化学生物学研究必不可少的肽合成仪

• 批准号: RTI-2021-00791
• 负责人: Boddy, Christopher
• 金额: $ 6.32万
• 依托单位: University of Ottawa
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=718787
• 关键词: Peptide; synthesizer; essential; natural; products

We desperately require a replacement solid phase peptide synthesizer. Access to peptides, including those substituted with non-proteinogenic amino acids, is essential for HQP in our research groups to characterize peptidic natural product biosynthetic pathways, study DNA-binding hydrocarbon stapled peptides, develop probes for labeling proteins, and to study enzymes in biological settings. Our previous solid phase peptide synthesizer, a CEM microwave peptide synthesizer, has been phased out by the manufacturer and both replacement and consumable parts are no longer available, leading to imminent loss of function for the instrument, thereby negatively impacting the training and progress through their degrees for all HQPs. No instrument is available on campus to replace this essential research tool. Without this infrastructure, the Boddy lab will not be able to maintain its internationally recognized, cutting edge research program on harnessing natural product biosynthesis to access complex molecules, nor will the Keillor lab be able to retain its position as a world leader in the development of chemical tools to interrogate transglutaminase (TGase) biology.

我们迫切需要一个替代的固相多肽合成器。获得多肽，包括那些被非蛋白生成氨基酸取代的多肽，对于HQP在我们的研究小组中表征多肽天然产物的生物合成途径，研究DNA结合的碳氢化合物结合多肽，开发标记蛋白质的探针，以及研究生物环境中的酶是至关重要的。我们之前的固相多肽合成器CEM微波多肽合成器已被制造商淘汰，不再提供更换部件和消耗品部件，导致仪器即将失去功能，从而对所有HQP的培训和进步产生负面影响。校园里没有任何仪器可以取代这一重要的研究工具。如果没有这些基础设施，Boddy实验室将无法维持其在利用天然产物生物合成来获取复杂分子方面的国际公认的尖端研究计划，Keillor实验室也将无法保持其在开发用于询问转谷氨酰胺酶(TGase)生物的化学工具方面的世界领先地位。