I2I Phase I: Reagentless sensor technologies for peripheral biochemical monitoring

I2I 第一阶段：用于外周生化监测的无试剂传感器技术

• 批准号: 561559-2021
• 负责人: Kelley, Shana
• 金额: $ 9.11万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Idea to Innovation
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=724259
• 关键词: I2I; Phase; Reagentless; sensor; technologies

Wearable sensor systems present a unique opportunity for the continual monitoring of vital signs and health status of an individual. These systems can be integrated into common items such as clothing, contact lenses, bandages, and watches to detect and quantify analytes from mucosal membranes or biological fluids. One major challenge to fully realize these systems is the development of platforms that allow for reagent-free analysis of complex biomolecules such as proteins, nucleic acids, and metabolites in real time. Electrochemical sensors are a powerful platform for environmental or biological monitoring based on inexpensive instrumentation and straightforward measurements of electrical currents. As small (nA) currents can be reliably measured, low detection thresholds are feasible. Several challenges remain for its implementation in practical devices and generalized for all types of protein analytes. We recently developed an enzyme-free and regent-free electrochemical reporter system that exhibits levels of sensitivity suitable for analysis of biological samples without the need for pre-processing. In this project we will integrate these electrochemical sensors into biocompatible probes for in situ biochemical monitoring. We will test the developed device in an animal model of diabetes demonstrating continual and reagent free monitoring of insulin levels from interstitial fluids. Requisite hardware and software for controlling the wearable monitoring device will be developed and tested in the project. The project deliverables will have a large impact on the Canadian population with potential impact in monitoring the health of providing continual biomarker monitoring in healthcare for chronic conditions.

可穿戴传感器系统为持续监测个体的生命体征和健康状态提供了独特的机会。这些系统可以集成到衣服、隐形眼镜、绷带和手表等常见物品中，以检测和量化粘膜或生物液体中的分析物。完全实现这些系统的一个主要挑战是开发允许真实的时间内对复杂生物分子（如蛋白质、核酸和代谢物）进行无试剂分析的平台。电化学传感器是一个强大的平台，用于环境或生物监测的基础上，廉价的仪器和电流的直接测量。由于可以可靠地测量小（nA）电流，因此低检测阈值是可行的。它在实际设备中的实现仍然存在一些挑战，并推广到所有类型的蛋白质分析物。我们最近开发了一种无酶和无试剂的电化学报告系统，该系统具有适用于生物样品分析的灵敏度水平，而无需预处理。在这个项目中，我们将这些电化学传感器集成到生物相容性探针，用于原位生化监测。我们将在糖尿病动物模型中测试所开发的设备，证明连续和无试剂监测间质液中的胰岛素水平。该项目将开发和测试控制可穿戴监测设备所需的硬件和软件。该项目的可交付成果将对加拿大人口产生重大影响，并对慢性病医疗保健中提供持续生物标志物监测的健康监测产生潜在影响。