Unraveling the diversity and biological activity of cyanobacteria natural products

揭示蓝藻天然产物的多样性和生物活性

• 批准号: RGPIN-2021-03825
• 负责人: McMullin, David
• 金额: $ 1.75万
• 依托单位: Carleton University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=742738
• 关键词: Unraveling; diversity; biological; activity; cyanobacteria

With over one-million lakes and access to upwards of 20% of global freshwater resources, Canada is the steward for a significant amount of the planet's drinking water. Over the last one hundred years, freshwater ecosystems (e.g., rivers, lakes and wetlands) have undergone widespread environmental change. Intense pressure on these ecosystems from population growth, pollution, increased temperatures, and habitat degradation are responsible for this change. A visible consequence of this environmental change is the increase in frequency and magnitude of toxic cyanobacteria blooms. Cyanobacteria blooms release mixtures of biologically active compounds into freshwater that negatively impact human and ecosystem health, wildlife, and local economies. Despite this, the chemistry, environmental concentrations, toxicological risks, and beneficial properties of most cyanobacteria chemicals are unknown. My research program will advance cyanobacteria chemistry using innovative strategies. Metabolomics-guided natural product discovery will target novel chemicals for structural characterizations. This will enable comprehensive exposure and biological activity assessments for poorly characterized cyanobacteria chemicals. Combining phenotype assays with metabolomics will determine the molecular modes of action for chemicals purified by my program. This research will mine cyanobacteria, an understudied reservoir of new chemistry, for novel chemicals with applications in agriculture and medicine. It will also support Canadian regulators that have the daunting task of managing increasingly stressed freshwater resources without understanding the diversity of chemicals released into the environment.

加拿大拥有超过100万个湖泊，可获得全球20%以上的淡水资源，是地球上大量饮用水的管家。在过去的一百年里，淡水生态系统（如河流、湖泊和湿地）经历了广泛的环境变化。人口增长、污染、气温升高和栖息地退化给这些生态系统带来的巨大压力是造成这种变化的原因。这种环境变化的一个明显后果是有毒蓝藻繁殖的频率和幅度的增加。蓝藻大量繁殖释放生物活性化合物的混合物到淡水中，对人类和生态系统健康、野生动物和当地经济产生负面影响。尽管如此，大多数蓝藻化学物质的化学、环境浓度、毒理学风险和有益特性都是未知的。我的研究项目将使用创新策略推进蓝藻化学。代谢组学指导的天然产物发现将针对新的化学物质进行结构表征。这将使全面的暴露和生物活性评估表征差的蓝藻化学物质。将表型分析与代谢组学相结合，将确定由我的程序纯化的化学物质的分子作用模式。这项研究将挖掘蓝藻，一个未充分研究的新化学水库，用于农业和医学应用的新化学品。它还将支持加拿大监管机构在不了解释放到环境中的化学物质多样性的情况下，管理日益紧张的淡水资源的艰巨任务。