Internal mechanics of whole muscle contraction

全肌肉收缩的内部机制

• 批准号: RGPIN-2020-07015
• 负责人: Wakeling, James
• 金额: $ 2.91万
• 依托单位: Simon Fraser University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=746233
• 关键词: Internal; mechanics; whole; muscle; contraction

Much of our understanding of muscle function comes from single fibre and isolated muscle experiments that commonly have steady contractile conditions and maximal activation, and muscle models reflect this. However, our muscles are normally used for unsteady and dynamic movements at submaximal levels of contraction. Contractions of whole muscles are more complex than that of their constituent muscle fibres. Indeed, recent models of whole muscle force production in vivo have large errors, indicating that we still have much to learn about how whole muscles contract. Not only do muscles develop force and change in length along their line of action, but forces and deformations occur across all three dimensions. Recently, the importance of the 3D structure of muscle, and the interactions between contractile and connective tissues have become evident. For example, compressing a muscle supresses the force developed in its line of action (up to 16%), and muscle contractions result in transverse work being done (tensing your glutes whilst you are sitting will raise your body due to transverse muscle work). Additionally, if you maximally activate a muscle then it will never shorten as fast as its constituent fibres are capable of, with this discrepancy (up to 35%) being greatest for large muscles at low levels of activation (typical conditions for our everyday movements). Different labs, including my own, have proposed factors to explain these phenomena, including internal elasticity, force transmission and transverse work, drag of inactive tissues, and inertial properties of the muscle tissue. However, we still lack a general framework that relates all these effects to a muscle's contractile performance. Identifying the mechanisms that contribute to whole-muscle function will increase our basic understanding of muscle biomechanics and enhance our ability to study and maintain animal and human mobility, agility and health. There is finite energy available for muscle contractions and so we propose that mechanisms that use internal energy, or even do external work in a transverse direction, will diminish the work output along its line of action. Not only that, but the efficiency of muscle contractions will be reduced in situations that incur a metabolic cost for using internal energy and doing transverse work. The objective of the proposed research is to use theoretical techniques, validated with cutting-edge experimental data, to relate the distribution of internal energy within a muscle to its 3D shape and force. In particular, we will refine and validate a finite element model of muscle contractions, investigate the relation between transverse compression and bulging on longitudinal force, and investigate the effect of muscle tissue mass on the dynamics and efficiency of muscle contractions. The long-term goal of my research program is to develop our mechanistic understanding of the contractile mechanics of whole muscle.

我们对肌肉功能的大部分理解来自单纤维和孤立肌肉实验，这些实验通常具有稳定的收缩条件和最大激活，肌肉模型反映了这一点。然而，我们的肌肉通常用于不稳定和动态运动在次最大水平的收缩。整个肌肉的收缩比其组成肌纤维的收缩更复杂。事实上，最近的活体肌肉力量产生模型存在很大的误差，这表明我们仍然需要了解整个肌肉如何收缩。 肌肉不仅会产生力量并沿着沿着它们的动作线改变长度，而且力量和变形会发生在所有三个维度上。最近，肌肉的3D结构以及收缩组织和结缔组织之间的相互作用的重要性已经变得明显。例如，压缩肌肉会抑制在其作用线上产生的力（高达16%），肌肉收缩会导致横向工作（当你坐着时拉紧你的臀部会因为横向肌肉工作而提升你的身体）。此外，如果你最大限度地激活一块肌肉，那么它永远不会像其组成纤维所能做到的那样快速缩短，这种差异（高达35%）对于低水平激活的大肌肉来说是最大的（我们日常运动的典型条件）。不同的实验室，包括我自己的实验室，提出了解释这些现象的因素，包括内部弹性，力传递和横向功，非活性组织的阻力，以及肌肉组织的惯性特性。然而，我们仍然缺乏一个通用的框架，将所有这些影响与肌肉的收缩性能联系起来。确定有助于整个肌肉功能的机制将增加我们对肌肉生物力学的基本理解，并提高我们研究和保持动物和人类移动性，敏捷性和健康的能力。 肌肉收缩的能量是有限的，因此我们提出，使用内部能量的机制，甚至在横向方向上做外部功，将减少沿着其作用线的功输出。不仅如此，但肌肉收缩的效率将降低的情况下，产生代谢成本使用内部能源和做横向工作。这项研究的目的是使用经过尖端实验数据验证的理论技术，将肌肉内部能量的分布与其3D形状和力量联系起来。特别是，我们将完善和验证肌肉收缩的有限元模型，研究横向压缩和膨胀之间的关系对纵向力，并研究肌肉组织质量对肌肉收缩的动力学和效率的影响。我的研究计划的长期目标是发展我们对整个肌肉收缩机制的机械理解。