Development of total consumption systems for inductively coupled plasma mass spectrometry in single particle and single cell modes

单颗粒和单细胞模式电感耦合等离子体质谱总消耗系统的开发

• 批准号: 578516-2022
• 负责人: Beauchemin, DianeD
• 金额: $ 2.19万
• 依托单位: Queen's University
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=747300
• 关键词: Development; total; consumption; systems; inductively

Metal nanoparticles are increasingly used in cosmetics, food packaging, textiles, toothpaste, and other ubiquitous products. These nanoparticles may end up in the potable water of rural and indigenous communities without water treatment plants, putting these populations at risk. The impact of these nanoparticles on our environment, our food, and our health is largely unknown because measuring nanoparticles in environmental and biological systems is very difficult. Similarly, measuring the uptake of metal-containing drugs by cells to develop smart therapeutics targeting tumors is also difficult because it requires analysis of numerous individual cells to assess the selectivity of the drug uptake by diseased cells versus healthy cells. This partnership aims to develop sample introduction systems (one for the analysis of nanoparticles, one for the analysis of cells) allowing the quick analysis of every nanoparticle or cell in each sample, which is not currently possible, to provide a complete picture. The systems to be developed are expected to facilitate the analysis of real samples, such as natural waters and other environmental samples by reducing or eliminating the sample pretreatment that is currently required with standard sample introduction systems, thereby increasing the number of samples that can be analyzed daily. The system that will be optimized for the analysis of cells will be used to assess the efficiency and selectivity of new drugs being designed by collaborators. Such analysis will allow drug dosage to be adjusted so that cancerous cells are preferentially targeted. It would in turn allow the advancement of copper- and nickel-containing targeted therapeutics to treat cancer and neurological disorders. A switch from platinum (currently used in anti-cancer drugs) to copper or nickel in therapeutic candidates would greatly benefit patients' access to more affordable (hence equitable) and safer medication. The intention is to patent the resulting systems and allow Burgener Research, a Canadian manufacturer of sample introduction systems, to sell them. Canada would then become a world leader in the measurement of nanoparticles and cells.

金属纳米颗粒越来越多地用于化妆品、食品包装、纺织品、牙膏和其他无处不在的产品。 这些纳米颗粒可能最终进入没有水处理厂的农村和土著社区的饮用水中，使这些人口处于危险之中。这些纳米颗粒对我们的环境、食物和健康的影响在很大程度上是未知的，因为测量环境和生物系统中的纳米颗粒非常困难。类似地，测量细胞对含金属药物的吸收以开发靶向肿瘤的智能疗法也很困难，因为它需要分析许多单个细胞以评估患病细胞与健康细胞对药物吸收的选择性。 该合作伙伴关系旨在开发样品引入系统（一个用于分析纳米颗粒，一个用于分析细胞），允许快速分析每个样品中的每个纳米颗粒或细胞，这是目前不可能的，以提供完整的图片。预计将开发的系统将通过减少或消除标准样品引入系统目前所需的样品预处理来促进真实的样品的分析，例如天然沃茨和其他环境样品，从而增加每天可以分析的样品数量。 该系统将被优化用于细胞分析，用于评估合作者设计的新药的效率和选择性。 这样的分析将允许调整药物剂量，从而优先靶向癌细胞。反过来，它将使含铜和镍的靶向治疗方法能够用于治疗癌症和神经系统疾病。 从铂（目前用于抗癌药物）转向铜或镍的治疗候选人将大大有利于患者获得更负担得起（因此公平）和更安全的药物。其目的是为由此产生的系统申请专利，并允许加拿大样品导入系统制造商Burgener Research销售这些系统。届时，加拿大将成为纳米颗粒和细胞测量领域的世界领先者。