Understanding and controlling complex modified electrochemical interfaces using novel measurements and model systems
使用新颖的测量和模型系统理解和控制复杂的改性电化学界面
基本信息
- 批准号:RGPIN-2022-04419
- 负责人:
- 金额:$ 3.5万
- 依托单位:
- 依托单位国家:加拿大
- 项目类别:Discovery Grants Program - Individual
- 财政年份:2022
- 资助国家:加拿大
- 起止时间:2022-01-01 至 2023-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The pandemic has highlighted the many unmet, or partially met, needs in society and in healthcare. The availability of effective, simple, accurate and sensitive bio-sensing capacities was realized as a critical part of the strategy for dealing with the pandemic and ensuing healthcare challenge going forward and for future challenges. Biosensors developed using electrochemical detection and measurement form an important part of the portable sensor ecosystem. They require sophisticated modification of the electrode surface so as to specifically detect the analyte of interest in complex media. Challenges in preparing these sensors for commercialization are many, but inevitably involve the modification and stability of the electrode surface, the quality of the surface modification and the characteristics of the underlying substrate. Understanding the interfacial characteristics at the molecular level while under measurement conditions (e.g., in buffer or in-situ) is required, though only a few techniques exist and more are needed. Surface analysis while in buffer is difficult and requires many complimentary approaches to achieve an unbiased result. Insight into the conditions of the surface-based probe or sensor transducer when in buffer, before or after binding the target is important for tailoring the design and surface engineering for the specific analytical need. Developing new in-situ surface analysis methods for characterizing real sensors will advance sensor development and commercialization. This research programme uses our unique in-situ fluorescence microscopy spectroelectrochemical methodology to study modified electrode surfaces and addresses many of the challenges above. The substrate used is a single crystal bead electrode that contains the complete stereographic triangle which provides access to all forms of the arrangement of the atoms that make up the underlying substrate. This electrode provides a unique insight into sensors that are typically prepared on poly-crystalline gold substrates (e.g., have a random variety of surface structures like grains separated by high energy grain boundaries). Understanding the role of these structural features has proven critical for improving sensor stability and sensing performance. The distribution and organization of the adsorbed molecules will be explored by developing single molecule optical methods. This will enable study of the local environment of the adsorbate, kinetics of single molecule recognition and the distribution of analyte binding efficiency. Advanced in-situ surface characterization will direct new methods for preparing the sensor surface achieving control over density, local environment and distribution of the adsorbates. This will improve sensor stability and performance. These results will be used to engineer surfaces to enable new detection modalities which will combine electrochemical and fluorescence methods in a dual detection scheme.
大流行凸显了社会和卫生保健领域许多未得到满足或部分得到满足的需求。提供有效、简单、准确和灵敏的生物感应能力是应对这一流行病及其带来的未来保健挑战和应对未来挑战战略的一个关键部分。利用电化学检测和测量技术开发的生物传感器是便携式传感器生态系统的重要组成部分。它们需要对电极表面进行复杂的修饰,以便在复杂的介质中特异性地检测感兴趣的分析物。在为商业化准备这些传感器的过程中存在许多挑战,但不可避免地涉及电极表面的修饰和稳定性,表面修饰的质量以及底层衬底的特性。在测量条件下(例如,在缓冲液或原位),了解分子水平上的界面特性是必要的,尽管只有少数技术存在,但需要更多的技术。表面分析,而在缓冲是困难的,需要许多互补的方法来实现无偏的结果。深入了解基于表面的探头或传感器传感器在缓冲、绑定目标之前或之后的情况,对于定制设计和表面工程以满足特定分析需求非常重要。开发新的原位表面分析方法来表征真实传感器将促进传感器的发展和商业化。该研究计划使用我们独特的原位荧光显微镜光谱电化学方法来研究修饰的电极表面,并解决上述许多挑战。所使用的衬底是一个单晶珠电极,包含完整的立体三角形,可以访问构成衬底的原子的所有形式的排列。该电极为通常在多晶金衬底上制备的传感器提供了独特的见解(例如,具有随机变化的表面结构,如由高能晶界分隔的颗粒)。了解这些结构特征的作用已被证明是提高传感器稳定性和传感性能的关键。通过发展单分子光学方法来探索吸附分子的分布和组织。这将有助于研究吸附物的局部环境、单分子识别动力学和分析物结合效率的分布。先进的原位表面表征将指导制备传感器表面的新方法,实现对吸附物密度、局部环境和分布的控制。这将提高传感器的稳定性和性能。这些结果将用于设计表面,以实现新的检测方式,将电化学和荧光方法结合在双重检测方案中。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Bizzotto, Dan其他文献
AFM and Cu Electrodeposition Studies of Reduced Graphene Oxide Modified Au(111) Facets Prepared using Electrodeposition and Post-Deposition Pulse Treatment
- DOI:
10.1149/1945-7111/ac2466 - 发表时间:
2021-09-01 - 期刊:
- 影响因子:3.9
- 作者:
Liu, Zhiguang;Grzedowski, Adrian Jan;Bizzotto, Dan - 通讯作者:
Bizzotto, Dan
Highly Sensitive Method to Isolate Photocurrent Signals from Large Background Redox Currents on Protein-Modified Electrodes
- DOI:
10.1002/celc.201900249 - 发表时间:
2019-06-03 - 期刊:
- 影响因子:4
- 作者:
Jun, Daniel;Beatty, J. Thomas;Bizzotto, Dan - 通讯作者:
Bizzotto, Dan
Tailoring the DNA SAM surface density on different surface crystallographic features using potential assisted thiol exchange
- DOI:
10.1016/j.electacta.2017.12.114 - 发表时间:
2018-01-20 - 期刊:
- 影响因子:6.6
- 作者:
Leung, Kaylyn K.;Gaxiola, Andrea Diaz;Bizzotto, Dan - 通讯作者:
Bizzotto, Dan
Controlling Nanoparticle Interconnectivity in Thin-Film Platinum Catalyst Layers
- DOI:
10.1021/acs.jpcc.6b04952 - 发表时间:
2016-09-29 - 期刊:
- 影响因子:3.7
- 作者:
Martens, Isaac;Pinaud, Blaise A.;Bizzotto, Dan - 通讯作者:
Bizzotto, Dan
On the Nature of DNA Self-Assembled Monolayers on Au: Measuring Surface Heterogeneity with Electrochemical in Situ Fluorescence Microscopy
- DOI:
10.1021/ja808696p - 发表时间:
2009-03-25 - 期刊:
- 影响因子:15
- 作者:
Murphy, Jeffrey N.;Cheng, Alan K. H.;Bizzotto, Dan - 通讯作者:
Bizzotto, Dan
Bizzotto, Dan的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Bizzotto, Dan', 18)}}的其他基金
Characterizing functionalized electrode surfaces with unique spectroelectrochemical methods for biosensor development
使用独特的光谱电化学方法表征功能化电极表面,用于生物传感器的开发
- 批准号:
RGPIN-2016-05528 - 财政年份:2021
- 资助金额:
$ 3.5万 - 项目类别:
Discovery Grants Program - Individual
Replacement of research grade potentiostat for advanced electrochemical research
替代研究级恒电位仪以进行高级电化学研究
- 批准号:
RTI-2022-00442 - 财政年份:2021
- 资助金额:
$ 3.5万 - 项目类别:
Research Tools and Instruments
Characterizing functionalized electrode surfaces with unique spectroelectrochemical methods for biosensor development
使用独特的光谱电化学方法表征功能化电极表面,用于生物传感器的开发
- 批准号:
RGPIN-2016-05528 - 财政年份:2020
- 资助金额:
$ 3.5万 - 项目类别:
Discovery Grants Program - Individual
Market assessment for "Detecting and quantifying opioids with a low-cost portable device for use by front line workers"
“使用供一线工作人员使用的低成本便携式设备检测和量化阿片类药物”的市场评估
- 批准号:
544501-2019 - 财政年份:2019
- 资助金额:
$ 3.5万 - 项目类别:
Idea to Innovation
Characterizing functionalized electrode surfaces with unique spectroelectrochemical methods for biosensor development
使用独特的光谱电化学方法表征功能化电极表面,用于生物传感器的开发
- 批准号:
RGPIN-2016-05528 - 财政年份:2019
- 资助金额:
$ 3.5万 - 项目类别:
Discovery Grants Program - Individual
Characterizing functionalized electrode surfaces with unique spectroelectrochemical methods for biosensor development
使用独特的光谱电化学方法表征功能化电极表面,用于生物传感器的开发
- 批准号:
RGPIN-2016-05528 - 财政年份:2018
- 资助金额:
$ 3.5万 - 项目类别:
Discovery Grants Program - Individual
Characterizing functionalized electrode surfaces with unique spectroelectrochemical methods for biosensor development
使用独特的光谱电化学方法表征功能化电极表面,用于生物传感器的开发
- 批准号:
RGPIN-2016-05528 - 财政年份:2017
- 资助金额:
$ 3.5万 - 项目类别:
Discovery Grants Program - Individual
Universal battery identification system
通用电池识别系统
- 批准号:
508334-2017 - 财政年份:2017
- 资助金额:
$ 3.5万 - 项目类别:
Engage Plus Grants Program
Characterizing functionalized electrode surfaces with unique spectroelectrochemical methods for biosensor development
使用独特的光谱电化学方法表征功能化电极表面,用于生物传感器的开发
- 批准号:
RGPIN-2016-05528 - 财政年份:2016
- 资助金额:
$ 3.5万 - 项目类别:
Discovery Grants Program - Individual
In-situ study of electrochemical interfaces modified by adsorbed organic molecules; characterizing heterogeneous adsorbate coverage with application to biosensors
吸附有机分子修饰电化学界面的原位研究;
- 批准号:
203356-2011 - 财政年份:2015
- 资助金额:
$ 3.5万 - 项目类别:
Discovery Grants Program - Individual
相似国自然基金
阴离子聚合速度及副反应控制机理及其用于(甲基)丙烯酸酯室温以上常规聚合的研究
- 批准号:50933002
- 批准年份:2009
- 资助金额:200.0 万元
- 项目类别:重点项目
混沌控制和同步中几个问题
- 批准号:10372054
- 批准年份:2003
- 资助金额:22.0 万元
- 项目类别:面上项目
相似海外基金
Understanding and Controlling the Cellular Forces that Coordinate Epithelial Tissue Morphogenesis
了解和控制协调上皮组织形态发生的细胞力
- 批准号:
10473590 - 财政年份:2021
- 资助金额:
$ 3.5万 - 项目类别:
Understanding and controlling the cellular fate of fluorine-modified biologics
了解和控制氟改性生物制品的细胞命运
- 批准号:
10439828 - 财政年份:2021
- 资助金额:
$ 3.5万 - 项目类别:
Understanding and Controlling the Cellular Forces that Coordinate Epithelial Tissue Morphogenesis
了解和控制协调上皮组织形态发生的细胞力
- 批准号:
10674731 - 财政年份:2021
- 资助金额:
$ 3.5万 - 项目类别:
Understanding and controlling the cellular fate of fluorine-modified biologics
了解和控制氟改性生物制品的细胞命运
- 批准号:
10275995 - 财政年份:2021
- 资助金额:
$ 3.5万 - 项目类别:
Understanding and Controlling Macrophage Behavior in Angiogenesis
了解和控制血管生成中的巨噬细胞行为
- 批准号:
10889772 - 财政年份:2016
- 资助金额:
$ 3.5万 - 项目类别:
Understanding and Controlling Macrophage Behavior in Angiogenesis
了解和控制血管生成中的巨噬细胞行为
- 批准号:
10296177 - 财政年份:2016
- 资助金额:
$ 3.5万 - 项目类别:
Understanding and Controlling Macrophage Behavior in Angiogenesis
了解和控制血管生成中的巨噬细胞行为
- 批准号:
10682565 - 财政年份:2016
- 资助金额:
$ 3.5万 - 项目类别:
Understanding and Controlling Macrophage Behavior in Angiogenesis
了解和控制血管生成中的巨噬细胞行为
- 批准号:
10471405 - 财政年份:2016
- 资助金额:
$ 3.5万 - 项目类别:
Understanding and Controlling p120 Dysfunction in CRC
了解和控制 CRC 中的 p120 功能障碍
- 批准号:
7245694 - 财政年份:2007
- 资助金额:
$ 3.5万 - 项目类别:
Understanding and Controlling p120 Dysfunction in CRC
了解和控制 CRC 中的 p120 功能障碍
- 批准号:
7620037 - 财政年份:
- 资助金额:
$ 3.5万 - 项目类别: