Characterizing functionalized electrode surfaces with unique spectroelectrochemical methods for biosensor development
使用独特的光谱电化学方法表征功能化电极表面,用于生物传感器的开发
基本信息
- 批准号:RGPIN-2016-05528
- 负责人:
- 金额:$ 3.35万
- 依托单位:
- 依托单位国家:加拿大
- 项目类别:Discovery Grants Program - Individual
- 财政年份:2016
- 资助国家:加拿大
- 起止时间:2016-01-01 至 2017-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Electrochemical processes form the basis of many new technologies (eg. batteries, fuel cells, solar cells) as well as approaches to bioanalytical sensing. The portability and small footprint of these sensors & their integration into smart phones will facilitate point of care analytical determinations, for which sensitive, specific and robust sensor development is required. Achieving this goal will require characterizing and controlling the functionalization of these surfaces which is at the core of this research program. We develop new methods to investigate these complex interfaces during electrochemical processes (in-situ).
Electrochemical based biosensors rely on the modification of the electrode surface with biologically active species (eg lipids, nucleic acids or enzymes) that can specifically interact with compounds of interest in the sample, and then transduce this interaction into a signal that can be measured accurately. This requires a high level of control over the preparation of the sensor surface. A biosensor must have a sufficient number of probes on the surface which are accessible by the targets in solution so that when specifically interacting, will create a change in the measured signal. Controlling the probe density, probe distribution, reducing the non-specific interactions (noise) by controlling the surface structures will enable improvement of the functionalized surfaces with extension to development of new biosensor transduction motifs.
Characterizing the electrochemical interface has been the focus of our research efforts for many years. We have developed a unique in-situ fluorescence microscopic method for analyzing these interfaces while controlling the electrochemical potential. This is accomplished by using a fluorophore labelled adsorbate. The fluorescence measured depends on the separation of the fluorophore from the electrode surface with fluorescence quenched when closer than 5nm. Our method enables a detailed understanding of the structure of these modified surfaces, from large scales (sub mm) to molecular sized regimes (10s of nm) and how this depends on the surface crystallography and the functionalization method (multiple step adsorption, co-adsorption, potential-controlled adsorption, chemical modification of the functionalized surface). We continue to advance our in-situ characterization method to include confocal microscopy with fluorescence lifetime imaging. These new analysis methods will be used in creating complex surfaces; from multi-component surfaces, to quantum dot decorated surfaces. Our long term goal is to devise methods for creating biosensor surfaces which are optimally functional guided by the use of advanced in-situ characterization techniques. This is the foundational principle which we will use to influence the creation of well defined biosensors and, more broadly all types of functionalized surfaces.
电化学过程构成了许多新技术的基础。电池,燃料电池,太阳能电池)以及生物分析传感方法。这些传感器的便携性和小占地面积及其与智能手机的集成将促进护理点分析确定,为此需要敏感,特定和强大的传感器开发。实现这一目标将需要表征和控制这些表面的功能化,这是本研究计划的核心。我们开发了新的方法来研究电化学过程中这些复杂的界面(原位)。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
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Bizzotto, Dan其他文献
AFM and Cu Electrodeposition Studies of Reduced Graphene Oxide Modified Au(111) Facets Prepared using Electrodeposition and Post-Deposition Pulse Treatment
- DOI:
10.1149/1945-7111/ac2466 - 发表时间:
2021-09-01 - 期刊:
- 影响因子:3.9
- 作者:
Liu, Zhiguang;Grzedowski, Adrian Jan;Bizzotto, Dan - 通讯作者:
Bizzotto, Dan
Highly Sensitive Method to Isolate Photocurrent Signals from Large Background Redox Currents on Protein-Modified Electrodes
- DOI:
10.1002/celc.201900249 - 发表时间:
2019-06-03 - 期刊:
- 影响因子:4
- 作者:
Jun, Daniel;Beatty, J. Thomas;Bizzotto, Dan - 通讯作者:
Bizzotto, Dan
Tailoring the DNA SAM surface density on different surface crystallographic features using potential assisted thiol exchange
- DOI:
10.1016/j.electacta.2017.12.114 - 发表时间:
2018-01-20 - 期刊:
- 影响因子:6.6
- 作者:
Leung, Kaylyn K.;Gaxiola, Andrea Diaz;Bizzotto, Dan - 通讯作者:
Bizzotto, Dan
Controlling Nanoparticle Interconnectivity in Thin-Film Platinum Catalyst Layers
- DOI:
10.1021/acs.jpcc.6b04952 - 发表时间:
2016-09-29 - 期刊:
- 影响因子:3.7
- 作者:
Martens, Isaac;Pinaud, Blaise A.;Bizzotto, Dan - 通讯作者:
Bizzotto, Dan
On the Nature of DNA Self-Assembled Monolayers on Au: Measuring Surface Heterogeneity with Electrochemical in Situ Fluorescence Microscopy
- DOI:
10.1021/ja808696p - 发表时间:
2009-03-25 - 期刊:
- 影响因子:15
- 作者:
Murphy, Jeffrey N.;Cheng, Alan K. H.;Bizzotto, Dan - 通讯作者:
Bizzotto, Dan
Bizzotto, Dan的其他文献
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{{ truncateString('Bizzotto, Dan', 18)}}的其他基金
Understanding and controlling complex modified electrochemical interfaces using novel measurements and model systems
使用新颖的测量和模型系统理解和控制复杂的改性电化学界面
- 批准号:
RGPIN-2022-04419 - 财政年份:2022
- 资助金额:
$ 3.35万 - 项目类别:
Discovery Grants Program - Individual
Characterizing functionalized electrode surfaces with unique spectroelectrochemical methods for biosensor development
使用独特的光谱电化学方法表征功能化电极表面,用于生物传感器的开发
- 批准号:
RGPIN-2016-05528 - 财政年份:2021
- 资助金额:
$ 3.35万 - 项目类别:
Discovery Grants Program - Individual
Replacement of research grade potentiostat for advanced electrochemical research
替代研究级恒电位仪以进行高级电化学研究
- 批准号:
RTI-2022-00442 - 财政年份:2021
- 资助金额:
$ 3.35万 - 项目类别:
Research Tools and Instruments
Characterizing functionalized electrode surfaces with unique spectroelectrochemical methods for biosensor development
使用独特的光谱电化学方法表征功能化电极表面,用于生物传感器的开发
- 批准号:
RGPIN-2016-05528 - 财政年份:2020
- 资助金额:
$ 3.35万 - 项目类别:
Discovery Grants Program - Individual
Market assessment for "Detecting and quantifying opioids with a low-cost portable device for use by front line workers"
“使用供一线工作人员使用的低成本便携式设备检测和量化阿片类药物”的市场评估
- 批准号:
544501-2019 - 财政年份:2019
- 资助金额:
$ 3.35万 - 项目类别:
Idea to Innovation
Characterizing functionalized electrode surfaces with unique spectroelectrochemical methods for biosensor development
使用独特的光谱电化学方法表征功能化电极表面,用于生物传感器的开发
- 批准号:
RGPIN-2016-05528 - 财政年份:2019
- 资助金额:
$ 3.35万 - 项目类别:
Discovery Grants Program - Individual
Characterizing functionalized electrode surfaces with unique spectroelectrochemical methods for biosensor development
使用独特的光谱电化学方法表征功能化电极表面,用于生物传感器的开发
- 批准号:
RGPIN-2016-05528 - 财政年份:2018
- 资助金额:
$ 3.35万 - 项目类别:
Discovery Grants Program - Individual
Characterizing functionalized electrode surfaces with unique spectroelectrochemical methods for biosensor development
使用独特的光谱电化学方法表征功能化电极表面,用于生物传感器的开发
- 批准号:
RGPIN-2016-05528 - 财政年份:2017
- 资助金额:
$ 3.35万 - 项目类别:
Discovery Grants Program - Individual
Universal battery identification system
通用电池识别系统
- 批准号:
508334-2017 - 财政年份:2017
- 资助金额:
$ 3.35万 - 项目类别:
Engage Plus Grants Program
In-situ study of electrochemical interfaces modified by adsorbed organic molecules; characterizing heterogeneous adsorbate coverage with application to biosensors
吸附有机分子修饰电化学界面的原位研究;
- 批准号:
203356-2011 - 财政年份:2015
- 资助金额:
$ 3.35万 - 项目类别:
Discovery Grants Program - Individual
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