Characterizing functionalized electrode surfaces with unique spectroelectrochemical methods for biosensor development

使用独特的光谱电化学方法表征功能化电极表面，用于生物传感器的开发

• 批准号: RGPIN-2016-05528
• 负责人: Bizzotto, Dan
• 金额: $ 3.35万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=645558
• 关键词: Characterizing; functionalized; electrode; surfaces; unique

Electrochemical processes form the basis of many new technologies (eg. batteries, fuel cells, solar cells) as well as approaches to bioanalytical sensing. The portability and small footprint of these sensors & their integration into smart phones will facilitate point of care analytical determinations, for which sensitive, specific and robust sensor development is required. Achieving this goal will require characterizing and controlling the functionalization of these surfaces which is at the core of this research program. We develop new methods to investigate these complex interfaces during electrochemical processes (in-situ). ***Electrochemical based biosensors rely on the modification of the electrode surface with biologically active species (eg lipids, nucleic acids or enzymes) that can specifically interact with compounds of interest in the sample, and then transduce this interaction into a signal that can be measured accurately. This requires a high level of control over the preparation of the sensor surface. A biosensor must have a sufficient number of probes on the surface which are accessible by the targets in solution so that when specifically interacting, will create a change in the measured signal. Controlling the probe density, probe distribution, reducing the non-specific interactions (noise) by controlling the surface structures will enable improvement of the functionalized surfaces with extension to development of new biosensor transduction motifs. ***Characterizing the electrochemical interface has been the focus of our research efforts for many years. We have developed a unique in-situ fluorescence microscopic method for analyzing these interfaces while controlling the electrochemical potential. This is accomplished by using a fluorophore labelled adsorbate. The fluorescence measured depends on the separation of the fluorophore from the electrode surface with fluorescence quenched when closer than 5nm. Our method enables a detailed understanding of the structure of these modified surfaces, from large scales (sub mm) to molecular sized regimes (10s of nm) and how this depends on the surface crystallography and the functionalization method (multiple step adsorption, co-adsorption, potential-controlled adsorption, chemical modification of the functionalized surface). We continue to advance our in-situ characterization method to include confocal microscopy with fluorescence lifetime imaging. These new analysis methods will be used in creating complex surfaces; from multi-component surfaces, to quantum dot decorated surfaces. Our long term goal is to devise methods for creating biosensor surfaces which are optimally functional guided by the use of advanced in-situ characterization techniques. This is the foundational principle which we will use to influence the creation of well defined biosensors and, more broadly all types of functionalized surfaces. **

电化学过程构成了许多新技术（例如电池、燃料电池、太阳能电池）以及生物分析传感方法的基础。这些传感器的便携性和占地面积小以及它们与智能手机的集成将有助于现场分析测定，为此需要开发灵敏、特定和强大的传感器。实现这一目标需要表征和控制这些表面的功能化，这是该研究计划的核心。我们开发了新方法来研究电化学过程（原位）期间这些复杂的界面。 ***基于电化学的生物传感器依赖于用生物活性物质（例如脂质、核酸或酶）对电极表面进行修饰，这些生物活性物质可以与样品中感兴趣的化合物发生特异性相互作用，然后将这种相互作用转换成可以准确测量的信号。这需要对传感器表面的准备进行高度控制。生物传感器的表面必须有足够数量的探针，溶液中的目标可以接触到这些探针，以便在特异性相互作用时，会产生测量信号的变化。通过控制表面结构来控制探针密度、探针分布、减少非特异性相互作用（噪声）将能够改进功能化表面，并扩展到新生物传感器转导基序的开发。 ***多年来，表征电化学界面一直是我们研究工作的重点。我们开发了一种独特的原位荧光显微镜方法来分析这些界面，同时控制电化学势。这是通过使用荧光团标记的吸附物来实现的。测量的荧光取决于荧光团与电极表面的分离，当距离小于 5 nm 时荧光会猝灭。我们的方法可以详细了解这些改性表面的结构，从大尺度（亚毫米）到分子尺寸（数十纳米），以及这如何取决于表面晶体学和功能化方法（多步吸附、共吸附、电位控制吸附、功能化表面的化学改性）。我们继续改进我们的原位表征方法，包括具有荧光寿命成像的共焦显微镜。这些新的分析方法将用于创建复杂的表面；从多组分表面到量子点装饰表面。我们的长期目标是设计创建生物传感器表面的方法，通过使用先进的原位表征技术来实现最佳功能。这是我们将用来影响定义明确的生物传感器以及更广泛的所有类型的功能化表面的创建的基本原则。 **